机构：[1]Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China[2]Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China[3]Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing,[4]Department of Clinical Laboratory, Xuanwu Hospital, Capital Medical University, Beijing, China医技科室检验科首都医科大学宣武医院[5]The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China[6]Daqing Oilfield General Hospital, Daqing, Heilongjiang, China[7]Mudanjiang First People’s Hospital, Heilongjiang, Mudanjiang, China[8]Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan[9]Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan
Background/Purpose: There are limited studies on species distribution and suscep-tibility profiles of Aspergillus strains isolated from patients with otomycosis in China.Methods: A total of 69 confirmed Aspergillus species isolates were obtained from ear swabs of patients diagnosed with otomycosis from 2017 to 2018 in northern China. Identification of these Aspergillus isolates at the species level was performed using conventional morphological methods and MALDI-TOF MS in combination with molecular sequencing, and in vitro suscepti-bility to nine antifungal agents was evaluated using the Sensititre YeastOne system.Results: The Aspergillus section Nigri had the greatest distribution of Aspergillus isolates. A. welwitschiae (n = 25) was the most predominant isolate in section Nigri, followed by A. tu-bingensis (n = 12) and A. niger (n = 11). Other Aspergillus species were also isolated, including A. terreus (n = 11), A. flavus/A. oryzae (n = 8), and A. fumigatus (n = 2). Ampho-tericin B, posaconazole, and echinocandins were highly in vitro active against all the isolates tested. 2.9% (2/69) of the isolates were resistant to azoles in our study, including one A. niger isolate with a high MIC value for itraconazole (ITR) (16 mg/L) and one A. tubingensis isolate cross-resistant to both voriconazole (VOR) (MIC >8 mg/L) and ITR (MIC >16 mg/L). One A. wel-witschiae and one A. niger isolate both had increased MIC values of 4 mg/L against VOR.Conclusions: A. welwitschiae was the most prevalent Aspergillus species isolated from pa-tients with otomycosis. Our findings also indicated that the azole-resistant Aspergillus section Nigri should be utilized to guide clinical medication for Otomycosis.Copyright (c) 2021, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
基金：
National Major Science and Technology Project for the Control and Prevention of Major Infectious Diseases of China [2018ZX10712001]; Beijing Key Clinical Specialty for Laboratory Medicine-Excellent Project [ZK201000]
第一作者机构：[1]Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China[2]Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China[3]Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China[2]Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China[9]Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan[*1]Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Number 1, Shuaifuyuan Road, Dongcheng District, Beijing, China[*2]Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, Number 7, Chung Shan South Road, Taipei, 100, Taiwan.
推荐引用方式(GB/T 7714)：
Jing Ran,Yang Wen-Hang,Xiao Meng,et al.Species identification and antifungal susceptibility testing of Aspergillus strains isolated from patients with otomycosis in northern China[J].JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION.2022,55(2):282-290.doi:10.1016/j.jmii.2021.03.011.
APA：
Jing, Ran,Yang, Wen-Hang,Xiao, Meng,Li, Ying,Zou, Gui-Ling...&Hsueh, Po-Ren.(2022).Species identification and antifungal susceptibility testing of Aspergillus strains isolated from patients with otomycosis in northern China.JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION,55,(2)
MLA：
Jing, Ran,et al."Species identification and antifungal susceptibility testing of Aspergillus strains isolated from patients with otomycosis in northern China".JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION 55..2(2022):282-290
