机构:[1]Department of Neurosurgery, Xuanwu Hospital Capital Medical University神经科系统神经外科首都医科大学宣武医院[2]China Clinical Research Center for Epilepsy Capital Medical University[3]China Beijing Municipal Geriatric Medical Research Center
The amygdala is one of the most common origins of seizures, and the amygdala mouse model is essential for the illustration of epilepsy. However, few studies have described the experimental protocol in detail. This paper illustrates the whole process of amygdala electrical kindling epilepsy model making, with the introduction of a method of bipolar electrode fabrication. This electrode can both stimulate and record, reducing brain injury caused by implanting separate electrodes for stimulation and recording. For long-term electroencephalogram (EEG) recording purposes, slip rings were used to eliminate the record interruption caused by cable tangles and falling off. After periodic stimulation (60 Hz, 1 s every 15 min) of the basolateral amygdala (AP: 1.67 mm, L: 2.7 mm, V: 4.9 mm) for 19.83 ± 5.742 times, full kindling was observed in six mice (defined as induction of three continuous grade V episodes classified by Racine's scale). An intracranial EEG was recorded throughout the entire kindling process, and an epileptic discharge in the amygdala lasting 20-70 s was observed after kindling. Therefore, this is a robust protocol for modeling epilepsy originating from the amygdala, and the method is suitable for revealing the role of the amygdala in temporal lobe epilepsy. This research contributes to future studies on the mechanisms of mesial temporal lobe epilepsy and novel antiepileptogenic drugs.
基金:
The research was supported by the National Natural Science
Foundation of China (No. 82030037, 81871009) and Beijing
Municipal Health Commission (11000022T000000444685).
第一作者机构:[1]Department of Neurosurgery, Xuanwu Hospital Capital Medical University[2]China Clinical Research Center for Epilepsy Capital Medical University
共同第一作者:
通讯作者:
通讯机构:[1]Department of Neurosurgery, Xuanwu Hospital Capital Medical University[2]China Clinical Research Center for Epilepsy Capital Medical University[3]China Beijing Municipal Geriatric Medical Research Center
推荐引用方式(GB/T 7714):
Lu Yongchang,Dai Yang,Ou Siqi,et al.Using a Bipolar Electrode to Create a Temporal Lobe Epilepsy Mouse Model by Electrical Kindling of the Amygdala[J].JOVE-JOURNAL OF VISUALIZED EXPERIMENTS.2022,(184):doi:10.3791/64113.
APA:
Lu Yongchang,Dai Yang,Ou Siqi,Miao Yujing,Wang Ying...&Zhao Guoguang.(2022).Using a Bipolar Electrode to Create a Temporal Lobe Epilepsy Mouse Model by Electrical Kindling of the Amygdala.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,,(184)
MLA:
Lu Yongchang,et al."Using a Bipolar Electrode to Create a Temporal Lobe Epilepsy Mouse Model by Electrical Kindling of the Amygdala".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS ..184(2022)