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Proteomics analysis indicates the involvement of immunity and inflammation in the onset stage of SOD1-G93A mouse model of ALS

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收录情况： ◇ SCIE

作者：
Chen Lin[1,2] * ; Wang Ningyuan[3] ; Zhang Yingzhen[1Li Dongxiao[1] ; He Caili[1] ; Li Zhongzhong[1] ; Zhang Jian[4] ; Guo Yansu[2,5,*1] ;
机构： [1]Department of Neurology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, Hebei 050000, China [2]Beijing Geriatric Healthcare Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, China [3]Xiangya School of Medicine, Central South University, No.172 Tongzipo Road, Changsha, Hunan 410013, China [4]Department of Geriatrics, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, Hebei 050000, China [5]Beijing Municipal Geriatric Medical Research Center, No. 45 Changchun Street, Xicheng District, Beijing 100053, China
出处：
DOI： 10.1016/j.jprot.2022.104776
ISSN：

关键词： Amyotrophic lateral sclerosis 4D label-free quantitative proteomics Parallel reaction monitoring Immunity and inflammation SOD1-G93A mouse Lumbar spinal cord

摘要：
Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron degenerative disease, and the pathogenic mechanism that underlies ALS is still unclear. We analyzed the differentially expressed proteins (DEPs) in the spinal cord between SOD1-G93A transgenic mice at the onset stage and non-transgenic (NTG) littermates based on 4D label-free quantitative proteomics (4D-LFQ) with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In our study, 189 DEPs were screened, of which 166 were up-regulated and 23 down-regulated. Clusters of Orthologous Groups (COG)/ EuKaryotic Orthologous Groups (KOG) classification, subcellular localization annotation, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, clustering analysis and protein-protein interaction (PPI) network analyses were performed. Parallel reaction monitoring (PRM) analysis validated 48 proteins from immunity and inflammation-related pathways of KEGG. We described the function and distribution of DEPs, most of which were involved in the following pathways: complement and coagulation cascades, antigen processing and presentation, NF-kappa B signaling pathway, Retinoic acid-inducible gene I (RIG) -I-like receptor signaling pathway, the extracellular matrix-receptor (ECM-receptor) interaction, focal adhesion, phagosome and lysosome. PPI network analysis identified Fn1, Fga, Serpina1e and Serpina3n as potential biomarkers. Our discoveries broaden the view and expand our understanding of immunity and inflammation in ALS. SIGNIFICANCE: This study gives a comprehensive description of DEPs in the spinal cord proteomics of SOD1-G93A mice at the onset period. Compared with a previous study focusing on progressive stage, we showed that immunity and inflammation play an important role at the onset stage of ALS. Several pathways validated by PRM bring new insight to the pathological mechanisms of ALS. The participation of RIG-I-like signaling pathway in ALS and potential biomarkers Fga, Fn1, Serpina1e and Serpina3n are supplements to existing knowledge.Copyright © 2022. Published by Elsevier B.V.

基金：
National Natural Science Foundation of China, China (grant no. 81971200) and Beijing Municipal Natural Science Foundation, China (grant no. 7222082).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类 | 2 区 生物学
小类 | 2 区 生化研究方法
最新[2025]版：
大类 | 3 区 生物学
小类 | 2 区 生化研究方法
JCR分区：
出版当年[2021]版：
Q2 BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q2 BIOCHEMICAL RESEARCH METHODS

影响因子： 最新[2023版] 最新五年平均 出版当年[2021版] 出版当年五年平均 出版前一年[2020版] 出版后一年[2022版]

第一作者：
第一作者机构： [1]Department of Neurology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, Hebei 050000, China [2]Beijing Geriatric Healthcare Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, China
通讯作者：
通讯机构： [2]Beijing Geriatric Healthcare Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, China [5]Beijing Municipal Geriatric Medical Research Center, No. 45 Changchun Street, Xicheng District, Beijing 100053, China [*1]Beijing Geriatric Healthcare Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng, Beijing 100053,China
推荐引用方式(GB/T 7714)：
Chen Lin,Wang Ningyuan,Zhang Yingzhen,et al.Proteomics analysis indicates the involvement of immunity and inflammation in the onset stage of SOD1-G93A mouse model of ALS[J].JOURNAL OF PROTEOMICS.2023,272:doi:10.1016/j.jprot.2022.104776.
APA：
Chen Lin,Wang Ningyuan,Zhang Yingzhen,He Caili,Li Zhongzhong...&Guo Yansu.(2023).Proteomics analysis indicates the involvement of immunity and inflammation in the onset stage of SOD1-G93A mouse model of ALS.JOURNAL OF PROTEOMICS,272,
MLA：
Chen Lin,et al."Proteomics analysis indicates the involvement of immunity and inflammation in the onset stage of SOD1-G93A mouse model of ALS".JOURNAL OF PROTEOMICS 272.(2023)

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