机构：[1]Biomedical Information Center, Beijing You'An Hospital, Capital Medical University, Beijing 100069, China.[2]Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.[3]Department of Clinical Laboratory, Xuanwu Hospital, National Clinical Research Center for Geriatric Diseases, Capital Medical University, Beijing 100053, China.医技科室检验科首都医科大学宣武医院[4]Beijing Institute of Hepatology, Beijing You'An Hospital, Capital Medical University, Beijing 100069, China.[5]Department of Hepatobiliary Surgery, Beijing You'An Hospital, Capital Medical University, Beijing 100069, China.
Neuroblast differentiation-associated protein AHNAK, a large structural scaffold protein, remains mysterious in biological processes. AHNAK plays a suppressive or progressive role in different types of cancers. To investigate the role of the AHNAK in hepatocellular carcinoma (HCC), cell viability assays were performed to determine the cell proliferation of the stable AHNAK-knockdown HepG2 cell line; co-immunoprecipitation (Co-IP) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) were performed on HCC and matched paracancerous (MPC) tissues. The Metascape platform was used for enrichment analyses; the "ComplexHeatmap" package was applied for cluster analyses and visualization. Co-IP, Western botting and immunofluorescence double staining were performed to assess the interactions between AHNAK and insulin-like growth factor 1 receptor (IGF-1R). AHNAK silencing reduced the viability of HepG2 cells; the interactome in HCC and MPC tissues enriched 204 pathways and processes, which partially reflected the signature of HCC field cancerization. AHNAK could co-localize and interact with IGF-1R. These results suggested that the AHNAK complex contributes to HCC growth, potentially by interacting with IGF-1R.
基金：
This research was funded by Beijing Natural Science Foundation (7191004), Capital’s Funds
of Health Improvement and Research (CFH2020-1-2182), Beijing You’An Hospital (BJYAHKF2021003,
YNKTQN2021007), Beijing Key Laboratory (BZ0373), “Sail Plan” from Beijing Hospital Authority
(XMLX201711), Beijing Municipal Science & Technology Commission (Z171100001017078) and
National Key Research and Development Project (2020YFE0202400).
第一作者机构：[1]Biomedical Information Center, Beijing You'An Hospital, Capital Medical University, Beijing 100069, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Li Kang,Song Ke,Hou Yuli,et al.AHNAK Contributes to Hepatocellular Carcinoma Growth by Interacting with IGF-1R[J].MOLECULES.2022,27(24):doi:10.3390/molecules27248680.
APA：
Li Kang,Song Ke,Hou Yuli,Tian Yuan,Wang Huijuan...&Zhang Yonghong.(2022).AHNAK Contributes to Hepatocellular Carcinoma Growth by Interacting with IGF-1R.MOLECULES,27,(24)
MLA：
Li Kang,et al."AHNAK Contributes to Hepatocellular Carcinoma Growth by Interacting with IGF-1R".MOLECULES 27..24(2022)
