Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats
机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, And Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, China内科系统科技平台老年医学科神经变性病教育部重点实验室首都医科大学宣武医院[2]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medical, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[3]College of Pharmacy, Jilin University, Changchun, Jilin 130021, China[4]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China[5]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[6]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China
Neural stem cells (NSCs) and derivatives are potential cellular sources to treat neurological diseases. In the current study, we reprogrammed human peripheral blood mononuclear cells into induced NSCs (iNSCs) and inserted GFP gene into the AAVS1 site for graft tracing. Targeted integration of GFP does not affect the proliferation and differentiation capacity of iNSCs. iNSC-GFP can be further differentiated into dopaminergic precursors (DAPs) and motor neuron precursors (MNPs), respectively. iNSCs were engrafted into the motor cortex and iNSC-DAPs into the striatum and substantia nigra (SN) of a nonhuman primate, respectively. The surviving iNSCs could respond to the microenvironment of the cortex and spontaneously differentiate into mature neurons that extended neurites. iNSC-DAPs survived well and matured into DA neurons following transplantation into the striatum and SN. iNSC-MNPs could also survive and turn into motor neurons after being engrafted into the spinal cord of rats. The results suggest that iNSCs and derivatives have a potential to be used for the treatment of neurological diseases.
基金：
Stem Cell and Translation National Key Project [2016YFA0101403]; National Natural Science Foundation of China [82171250, 81973351]; Beijing Municipal Natural Science Foundation [5142005]; Beijing Talents Foundation [2017000021223TD03]; Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [TCD20180333]; Beijing Municipal Health Commission Fund [PXM2020_026283_000005]; Ten Thousand Talents Fund [2018A03]; Royal Society-Newton Advanced Fellowship [NA150482]; Beijing One Hundred, Thousand; Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan (CIT)
第一作者机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, And Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, And Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, China[2]State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medical, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[4]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China[5]Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China[6]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China
推荐引用方式(GB/T 7714)：
Li Mengjia,Wang Zhengbo,Zheng Tianqi,et al.Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats[J].STEM CELLS INTERNATIONAL.2022,2022:1396735.doi:10.1155/2022/1396735.
APA：
Li, Mengjia,Wang, Zhengbo,Zheng, Tianqi,Huang, Tianzhuang,Liu, Baoguo...&Chen, Zhiguo.(2022).Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats.STEM CELLS INTERNATIONAL,2022,
MLA：
Li, Mengjia,et al."Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats".STEM CELLS INTERNATIONAL 2022.(2022)
