机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Departments of Neurosurgery, The Second Clinical Medical School of Inner Mongolia University for Nationalities, Hulunbuir, Inner Mongolia 022150[3]Departments of Hematology and Oncology, The Second Clinical Medical School of Inner Mongolia University for Nationalities, Hulunbuir, Inner Mongolia 022150[4]Department of Neurosurgery, Hulunbeier People's Hospital, Hulunbuir, Inner Mongolia 021000, P.R. China
The effect of intravascular interventional embolization and surgical operation on matrix metalloprotein (MMP)-2, MMP-9 and caspase3 in serum of intracranial aneurysms was analyzed to study the mechanisms. Seventy-nine patients of intracranial aneurysms from September 2011 to August 2014 were divided into the intervention group (n=41) and the craniotomy group (n=38) based on treatment methods, and 40 cases of normal volunteers with normal physical examination were selected as the control group. Patients in the intervention group were treated with intravascular interventional embolization, and the surgical group was treated with craniotomy and no treatment was performed in the control group. The survival rate and adverse reaction rate were calculated. ELISA kit was used to detect the level of reactive oxygen species (ROS), interleukin-6 (IL-6), tumor necrosis factor (TNF-) and IL-10 in serum; the mRNA and protein expression levels of MMP-2, MMP-9 and caspase3 in serum were detected by qPCR and western blotting; the protein expression levels of p-adenosine 5-monophosphate (AMP)-activated protein kinase (AMPK), Bcl-2 and Bax were also detected. The survival rate of patients in the intervention group was significantly higher than that in the craniotomy group (P<0.05). The ROS level in the intervention and craniotomy groups was significantly higher than that in the control group (P<0.01), while the levels of IL-6 and TNF- in the serum of the intervention group were lower than those in the craniotomy group (P<0.01). The mRNA and protein expression of MMP-2, MMP-9 and caspase3 of the intervention group was significantly lower than that of the craniotomy group (P<0.01). The protein expression of p-AMPK and Bcl-2/Bax in the intervention group were lower than that in the craniotomy group (P<0.01). Intravascular interventional surgery can significantly increase the patient's survival time, and effectively reduce the expression of MMP-2, MMP-9 and caspase3 in the serum of intracranial aneurysm patients. The mechanism may be through the impact of ROS levels, thereby affecting the p-AMPK and Bcl2/Bax expression.
第一作者机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Departments of Neurosurgery, The Second Clinical Medical School of Inner Mongolia University for Nationalities, Hulunbuir, Inner Mongolia 022150
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Hulunbeier People's Hospital,20 Shengli Avenue, Hulunbuir, Inner Mongolia 021000, P.R. China
推荐引用方式(GB/T 7714)：
LIANG ZOU,YANYAN HOU,BAIXIANG YU,et al.The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2018,16(6):4511-4518.doi:10.3892/etm.2018.6740.
APA：
LIANG ZOU,YANYAN HOU,BAIXIANG YU,SHUANG LI&YANLI DU.(2018).The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients.EXPERIMENTAL AND THERAPEUTIC MEDICINE,16,(6)
MLA：
LIANG ZOU,et al."The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients".EXPERIMENTAL AND THERAPEUTIC MEDICINE 16..6(2018):4511-4518
