机构：[1]Department of Respiratory Medicine, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, PR China[2]Department of Respiratory Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang City, Guizhou Province, PR China[3]Department of Endocrinology, Affiliated Hospital of Guizhou Medical University, Guiyang City, Guizhou Province, PR China
Aim: The airway epithelium of smokers exhibits upregulated SPRR3, an indicator of pathogenic keratinization. The mechanisms underlying this phenomenon require investigation. Patients & methods: Human bronchial epithelial (HBE) SPRR3 expression was analyzed by smoking status. Primary HBE cells were exposed to cigarette smoke (CS). SPRR3 expression, SPRR3 promoter activity, AP-1 factor binding and AP-1 factors' effects were analyzed. Results: Current smokers display SPRR3 upregulation relative to never smokers. CS upregulates SPRR3 transcription in an exposure-dependent manner. CS promotes c-Jun and Fra1 binding to the SPRR3-AP-1/TRE site. Wild-type c-Jun and Fra1 upregulate, whereas c-Jun and Fra1, dominant-negative mutants, suppress SPRR3 promoter activity. Conclusion: CS induces SPRR3 upregulation in HBE cells by promoting aberrant c-Jun/Fra1 dimerization.
基金：
This work was supported by the Chinese Medical Association’s Clinical Research and Special Project Funds (grant number:
12020130273) and the Provincial Governor’s Special Fund of Guizhou Province (grant number: 2012100).
第一作者机构：[1]Department of Respiratory Medicine, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, PR China[2]Department of Respiratory Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang City, Guizhou Province, PR China
通讯作者：
通讯机构：[1]Department of Respiratory Medicine, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, PR China[3]Department of Endocrinology, Affiliated Hospital of Guizhou Medical University, Guiyang City, Guizhou Province, PR China
推荐引用方式(GB/T 7714)：
Xiwei Hu,Nianchun Peng,FeiQi,et al.Cigarette smoke upregulates SPRR3 by favoring c-Jun/Fra1 heterodimerization in human bronchial epithelial cells[J].FUTURE ONCOLOGY.2018,14(25):2599-2613.doi:10.2217/fon-2018-0043.
APA：
Xiwei Hu,Nianchun Peng,FeiQi,Jingwen Li,Lixin Shi&Rui Chen.(2018).Cigarette smoke upregulates SPRR3 by favoring c-Jun/Fra1 heterodimerization in human bronchial epithelial cells.FUTURE ONCOLOGY,14,(25)
MLA：
Xiwei Hu,et al."Cigarette smoke upregulates SPRR3 by favoring c-Jun/Fra1 heterodimerization in human bronchial epithelial cells".FUTURE ONCOLOGY 14..25(2018):2599-2613
