机构：[1]Department of Pathology, Medical College of Soochow University, Suzhou, China[2]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou, China[3]Department of Bioinformatics, Medical College of Soochow University, Soochow University, Suzhou, China[4]Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi, China[5]Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, China
MiRNA isoforms (isomiRs) were defined as an addition or deletion of one or more nucleotides at the 5 or 3 ends or both. Different isomiRs of the same miRNA can target different genes, which have extended the regulatory scale medicated by miRNA. In this study, we systematically analyzed miRNA isoforms in hepatocellular carcinoma (HCC) based on The Cancer Genome Atlas (TCGA) data and further explore their role by in silico and in vitro studies. We found that higher variety and quantity of miR-139-5p isoforms negatively correlated with the malignancy of HCC. And patients with higher variety and quantity of iso-miR-139-5p exhibited favorable survival, independent of tumor stage. Interestingly, miR-139-5p -1|-1 showed increased complementary effect of its target IGF1R than the archetype of miR-139-5p, and could further inhibit cellular movement more vigorously than its archetype. In conclusion, not only miR-139-5p itself, but its isoforms' variety and quantity confer suppressive role in HCC.
基金：
Natural Science Foundation of Jiangsu
Province, Grant number: BE2016666;
National Natural Science Foundation of
China, Grant numbers: 31471189,
81402724; Postgraduate Research &
Practice Innovation Program of Jiangsu
Province, Grant number: KYCX17_2032
第一作者机构：[1]Department of Pathology, Medical College of Soochow University, Suzhou, China
通讯作者：
通讯机构：[*1]Department of Pathology, Soochow University, Suzhou 215123, China.
推荐引用方式(GB/T 7714)：
Hengli Ni,Xiaoxiao Dai,Xueqin Leng,et al.Higher variety and quantity of microRNA-139-5p isoforms confer suppressive role in hepatocellular carcinoma[J].JOURNAL OF CELLULAR BIOCHEMISTRY.2018,119(8):6806-6813.doi:10.1002/jcb.26874.
APA：
Hengli Ni,Xiaoxiao Dai,Xueqin Leng,Min Deng,Yan Qin...&Yao Liu.(2018).Higher variety and quantity of microRNA-139-5p isoforms confer suppressive role in hepatocellular carcinoma.JOURNAL OF CELLULAR BIOCHEMISTRY,119,(8)
MLA：
Hengli Ni,et al."Higher variety and quantity of microRNA-139-5p isoforms confer suppressive role in hepatocellular carcinoma".JOURNAL OF CELLULAR BIOCHEMISTRY 119..8(2018):6806-6813
