机构：[a]Department of Plastic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China[b]Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
The present study examined expression and potential functions of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in human skin squamous cell carcinoma (SCC). We show that IGF2BP1 mRNA and protein expression levels were upregulated in established (A431 line) and primary human skin SO: cells. Its expression was also increased in human skin SCC tissues, as compared to the normal skin tissues: In skin SCC cells, IGF2BP1 silencing or CRISPR/Cas9 knockout decreased levels of IGF2BP1-stablized mRNAs, including IGF2, CD44, Glil and Myc. Furthermore, skin SCC cell survival and proliferation were inhibited by:IGF2BP1 silencing/knockout. Conversely, forced over-expression of IGF2BP1 further promoted A431 cell. survival and proliferation. Furthermore, siRNA-mediated knockdown of IGF2BP1-bound long non-coding RNA THOR ("Lnc-THOR") similarly depleted IGF2BP1-dependent mRNAs, causing inhibition on A431 cell survival and proliferation. In vivo, IGF2BP1 silencing or knockout inhibited A431 tumor xenograft growth in mice. Together, we conclude that IGF2BP1 over-expression in skin SCC cells is essential for cell growth. (C) 2018 Elsevier Inc. All rights reserved.
基金：
This study was supported in part by the Natural science Foundation
of Jiangsu Province (BK20160340), and by the Second Affiliated
Hospital of Soochow University Preponderant Clinic
Discipline Group Project (XKQ2015003).
第一作者机构：[a]Department of Plastic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China[b]Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
通讯作者：
通讯机构：[*1]Department of Plastic surgery, the Second Affiliated Hospital of Soochow University. San-xiang Rd, Suzhou, Jiangsu, 215004, China.
推荐引用方式(GB/T 7714)：
Zhengjun Liu,Guangyu Wu,Cai Lin,et al.IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,501(3):731-738.doi:10.1016/j.bbrc.2018.05.057.
APA：
Zhengjun Liu,Guangyu Wu,Cai Lin,Hailei Guo,Jianjun Xu&Tianlan Zhao.(2018).IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,501,(3)
MLA：
Zhengjun Liu,et al."IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 501..3(2018):731-738
