机构：[1]Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Soochow University, Suzhou, China,[2]Department of Oncology, the Second Affiliated Hospital of Soochow University, Suzhou, China,[3]Department of Molecular Medicine Center, the Second Affiliated Hospital of Soochow University, Suzhou, China,[4]Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China
CA125 amounts have a large overlap in ovarian cancer and benign diseases. We conducted a retrospective cohort trial to assess the clinical value of circulating cell-free DNA concentration and integrity index for the diagnosis of ovarian cancer. A total of 150 patients were recruited. Plasma samples of 24 ovarian cancer patients, 12 benign ovarian cysts, and 12 healthy controls were assessed. By amplifying short ALU-115 repeat and long ALU-219 fragments, circulating cell-free DNA concentrations and integrity index were measured. Plasma ALU-219 fragment levels and integrity index were significantly higher in the ovarian cancer group compared with the benign disease and healthy control groups (p = 0.023 and p = 0.004, respectively). These findings indicated that plasma ALU-219 levels and integrity may have a clinical value in the early diagnosis of ovarian cancer.
基金：
National Natural Science Foundation of China
(No. 81301267), the Suzhou Science and
Technology Development Program (SZS201618),
and the research foundation of the Second
Affiliated Hospital of Soochow University
(SDFEYQN201302) (S4 File).
第一作者机构：[1]Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Soochow University, Suzhou, China,
共同第一作者：
通讯作者：
通讯机构：[3]Department of Molecular Medicine Center, the Second Affiliated Hospital of Soochow University, Suzhou, China,[4]Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China
推荐引用方式(GB/T 7714)：
Rong Zhang,Wangyang PU,Shuyun Zhang,et al.Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial[J].PLOS ONE.2018,13(2):e0191756.doi:10.1371/journal.pone.0191756.
APA：
Rong Zhang,Wangyang PU,Shuyun Zhang,Li Chen,Weipei Zhu...&Kai Li.(2018).Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial.PLOS ONE,13,(2)
MLA：
Rong Zhang,et al."Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial".PLOS ONE 13..2(2018):e0191756
