机构：[1]Department of Neurology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China,[2]Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China,[3]Department of Ultrasound, Shanghai Tongji Hospital of Tongji University, Shanghai, China,[4]Department of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-jin Hospital, Shanghai Jiao-tong University School of Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai, China,[5]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and Institute of Neuroscience, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, China,[6]The Central Laboratory, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China
Ultrasound-targeted microbubble destruction (UTMD) and the herb medicine borneol can both facilitate the delivery of therapeutic agents to diseased brain regions and serve as promising adjuvant neuroprotective therapies. Our preliminary experiments showed that UTMD could exacerbate ischemic blood-brain barrier (BBB) opening, while borneol can protect the BBB. In this study, we tested the hypothesis that the combination of UTMD and borneol could attenuate UTMD-induced injury to the BBB under ischemic stroke conditions. Male albino mice were subjected to 60-min middle cerebral artery occlusion (MCAO) with reperfusion. Borneol and UTMD was given to mice 3 days before and 24 h after MCAO induction. BBB permeability, brain water contents, ultrastructural changes of the BBB and histopathological alterations were evaluated. Our data demonstrated that UTMD aggravated the leakage of Evans blue dye, ultrastructural alterations of cerebral microvasculature, brain edema, and even induced cerebral hemorrhage in ischemic stroke mice. Pretreatment with borneol significantly attenuated the above detrimental effects of UTMD on the BBB. This study indicates that under ischemic stroke conditions, the BBB becomes vulnerable to UTMD intervention, and the combination of borneol can help to maintain the integrity of the BBB.
基金：
This project was supported by grants from National Natural Science Foundation of China (81371328/81573750/81473491/ 81571149), the Young and Middle-Aged University Discipline Leaders of Zhejiang Province, China (2013277), Zhejiang Provincial Program for the Cultivation of High-level Health talents (2015), Priority Academic Program Development of Jiangsu Higher Education Institutions of China, Natural Science Foundation of Jiangsu Province of China (BK20151225), and Shenzhen Science and Technology Commission (JCYJ20170306093243010).
第一作者机构：[1]Department of Neurology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China,[2]Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China,[2]Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China,[6]The Central Laboratory, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China
推荐引用方式(GB/T 7714)：
Xiao-guang Zhang,Ye Song,Chang Shan,et al.Borneol attenuates Ultrasound-Targeted Microbubble Destruction-induced Blood-Brain Barrier Opening in Focal cerebral ischemia[J].FRONTIERS IN NEUROLOGY.2017,8(DEC):704.doi:10.3389/fneur.2017.00704.
APA：
Xiao-guang Zhang,Ye Song,Chang Shan,Xi-fan Wu,Yan-hua Tong...&Jie Liu.(2017).Borneol attenuates Ultrasound-Targeted Microbubble Destruction-induced Blood-Brain Barrier Opening in Focal cerebral ischemia.FRONTIERS IN NEUROLOGY,8,(DEC)
MLA：
Xiao-guang Zhang,et al."Borneol attenuates Ultrasound-Targeted Microbubble Destruction-induced Blood-Brain Barrier Opening in Focal cerebral ischemia".FRONTIERS IN NEUROLOGY 8..DEC(2017):704
