机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Department of Thoracic and Cardiovascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011[3]Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, P.R. China
Lung cancer is a leading cause of cancer-related mortalities worldwide. In the present study, a comparison of To determine the roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells expression levels between normal lung tissues and lung cancer tissues were compared using immunoblotting, and CCK-8 and Transwell assays. Lung cancer tissues had a decreased ARHGAP10 mRNA expression level compared to the adjacent normal tissues. The ectopic expression of ARHGAP10 significantly suppressed the migration, invasion and proliferation of lung cancer cells. Gene set enrichment analysis revealed that metastasis and Wnt signaling pathways were negatively correlated with ARHGAP10 expression. Immunoblotting analysis revealed that ARHGAP10 overexpression inhibited metastasis [matrix metalloproteinase (MMP)-2, MMP-9 and VEGF] and the expression of Wnt pathway-related proteins (beta-catenin and c-Myc). Moreover, the stimulation effects of lithium chloride, a GSK3 beta inhibitor, on the accumulation of beta-catenin were notably suppressed by ARHGAP10 overexpression. Collectively, ARHGAP10 acts to suppress tumor within lung cancer by affecting metastasis and Wnt signaling pathways. The results therefore suggest that ARHGAP10 is a potentially attractive target for the treatment of lung cancer.
第一作者机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Department of Thoracic and Cardiovascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011
通讯作者：
通讯机构：[*1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu 215004, P.R. China
推荐引用方式(GB/T 7714)：
JI-PING TENG,ZHI-YING YANG,YU-MING ZHU,et al.The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells[J].ONCOLOGY LETTERS.2017,14(4):4613-4618.doi:10.3892/ol.2017.6729.
APA：
JI-PING TENG,ZHI-YING YANG,YU-MING ZHU,DA NI,ZHI-JUN ZHU&XIAO-QIANG LI.(2017).The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells.ONCOLOGY LETTERS,14,(4)
MLA：
JI-PING TENG,et al."The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells".ONCOLOGY LETTERS 14..4(2017):4613-4618
