机构：[1]Department of Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[2]Department of Pathology, School of Biology ﹠ Basic Medical Sciences, Soochow University, Suzhou 215123, China[3]Department of Surgery, The People’s Hospital of Sihong County, Sihong 223900, Jiangsu Province, China[4]Laboratory Animal Research Center, Soochow University School of Medicine, Suzhou 215123, China[5]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[6]Laboratory of Molecular Pathology, Soochow University & Sihong County People’s Hospital, Suzhou 215123, China[7]Suzhou Key Laboratory of Tumor Microenvironment and Pathology, Soochow University, Suzhou 215006, China
Transforming growth factor-beta 1 is considered a key contributor to the progression of breast cancer. MicroRNAs are important factors in the development and progression of many malignancies. In the present study, upon studies of breast cancer cell lines and tissues, we showed that microRNA -196a-3p is decreased by transforming growth factor-beta 1 in breast cancer cells and associated with breast cancer progression. We identified neuropilin-2 as a target gene of microRNA -196a-3p and showed that it is regulated by transforming growth factor-beta 1. Moreover, transforming growth factor-beta 1-mediated inhibition of microRNA -196a-3p and activation of neuropilin-2were required for transforming growth factor-beta 1-induced migration and invasion of breast cancer cells. In addition, neuropilin-2 expression was suppressed in breast tumors, particularly in triple-negative breast cancers. Collectively, our findings strongly indicate that microRNA -196a-3p is a predictive biomarker of breast cancer metastasis and patient survival and a potential therapeutic target in metastatic breast cancer.
基金：
This study was supported by Jiangsu Provincial Commission of Health and Family Planning(H201517), the Chinese Nature Science Foundation (81272738, 81372867,81472297) and the A Project Funded by the Priority Academic Program Development of Jiangsu
Higher Education Institutions.
第一作者机构：[1]Department of Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[2]Department of Pathology, School of Biology ﹠ Basic Medical Sciences, Soochow University, Suzhou 215123, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Pathology, School of Biology ﹠ Basic Medical Sciences, Soochow University, Suzhou 215123, China[6]Laboratory of Molecular Pathology, Soochow University & Sihong County People’s Hospital, Suzhou 215123, China[7]Suzhou Key Laboratory of Tumor Microenvironment and Pathology, Soochow University, Suzhou 215006, China
推荐引用方式(GB/T 7714)：
Yan Chen,Shai Huang,Bo Wu,et al.Transforming growth factor-beta 1 promotes breast cancer metastasis by downregulating miR-196a-3p expression[J].ONCOTARGET.2017,8(30):49110-49122.doi:10.18632/oncotarget.16308.
APA：
Yan Chen,Shai Huang,Bo Wu,Jiankai Fang,Minsheng Zhu...&Shouli Wang.(2017).Transforming growth factor-beta 1 promotes breast cancer metastasis by downregulating miR-196a-3p expression.ONCOTARGET,8,(30)
MLA：
Yan Chen,et al."Transforming growth factor-beta 1 promotes breast cancer metastasis by downregulating miR-196a-3p expression".ONCOTARGET 8..30(2017):49110-49122
医学