机构：[1]National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China[2]School of Medicine, Shenzhen University, Shenzhen 518060, People’s Republic of China[3]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, People’s Republic of China[4]Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States
Detailed control of nano- and microstructures in porous biomaterial scaffold systems is important for control of interfacial and biological functions. Self-assembled silk protein nanostructured building blocks were incorporated into salt-leached scaffolds to control these features. Controllable concentration and pH were used to induce the formation of amorphous silk nanofibers in solution and also to reduce beta-sheet transformation during the more traditional salt-leaching process. These new scaffolds showed nanofibrous microporous structures, reduced beta-sheet content, and tunable mechanical properties. Bone marrow mesenchymal stem cells grew better and showed differentiation behavior on these nanofibrous scaffolds, suggesting cytocompatibility and support for tunable differentiation via the scaffolds. These results suggested a new strategy of designing bioactive silk scaffolds by combining traditional scaffold formation processes with the controllable self-assembly of silk.
基金：
The authors thank the National Basic Research Program of
China (973 Program, 2013CB934400), NSFC (81272106,
51403144), and the NIH (R01 DE017207, P41 EB002520).
The authors also thank the Natural Science Foundation of
Jiangsu Province (BK20140397, BK20140401) and the Jiangsu Provincial Special Program of Medical Science (BL2012004)
for support of this work.
第一作者机构：[1]National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China
推荐引用方式(GB/T 7714)：
Liying Xiao ,Shanshan Liu ,Danyu Yao ,et al.Fabrication of Silk Scaffolds with Nanomicroscaled Structures and Tunable Stiffness[J].BIOMACROMOLECULES.2017,18(7):2073-2079.doi:10.1021/acs.biomac.7b00406.
APA：
Liying Xiao,,Shanshan Liu,,Danyu Yao,,Zhaozhao Ding,,Zhihai Fan,...&David L. Kaplan.(2017).Fabrication of Silk Scaffolds with Nanomicroscaled Structures and Tunable Stiffness.BIOMACROMOLECULES,18,(7)
MLA：
Liying Xiao,,et al."Fabrication of Silk Scaffolds with Nanomicroscaled Structures and Tunable Stiffness".BIOMACROMOLECULES 18..7(2017):2073-2079
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