机构：[1]Department of Endocrinology, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, P.R. China.[2]Department of Endocrinology, Suzhou Science and Technology Town Hospital, Suzhou, 215000, P.R. China.[3]Department of Endocrinology, the East District of Suzhou Municipal Hospital, Suzhou, 215000, P.R. China.[4]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, 215123, P. R. China.
The present study was designed to investigate the roles of P2X3 receptors in dorsal root ganglion (DRG) neurons in colonic hypersensitivity and the effects of alpha-lipoic acid (ALA) on P2X3 receptor activity and colonic hypersensitivity of diabetic rats. Streptozotocin (STZ) was used to induce diabetic model. Abdominal withdrawal reflex (AWR) responding to colorectal distention (CRD) was recorded as colonic sensitivity. ATP-induced current density of colon-specific DRG (T13-L2 DRGs) neurons was measured with whole-cell patch clamp. The expression of P2X3Rs of T13-L2 DRGs was measured by western blot analysis. The results showed that AWR scores significantly increased after STZ injection. P2X3R expression and ATP current density of T13-L2 DRG neurons were enhanced in diabetic rats. Intraperitoneal injection with ALA once a day for 1 week remarkably reduced P2X3R expression and ATP current density in diabetic rats. Importantly, ALA treatment attenuated colonic hypersensitivity in diabetic rats. Our data suggest that STZ injection increases expression and function of P2X3 receptors of colon-specific DRG neurons, thus contributing to colonic hypersensitivity in diabetic rats. Administration of ALA attenuates diabetic colonic hypersensitivity, which is most likely mediated by suppressing expression and function of P2X3 receptors in DRGs of diabetic rats.
基金：
This work was supported by grants from National Natural Science Foundation of China (31400947 to HHZ,
81471041 to JH, 81230024 and 81471137 to GYX) and from the Science and Technology Development Program
of Suzhou (SYS201472 to JH). This work was also supported by grants from the Priority Academic Program
Development of Jiangsu Higher Education Institutions of China and from the preponderant clinic group project
of the Second Affiliated Hospital of Soochow University (XKQ2015001 and XKQ2015008). This work was also
supported by grants from the Jiangsu Youth Medical Talents Project (QNRC2016874 to HHZ).
第一作者机构：[1]Department of Endocrinology, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, P.R. China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Endocrinology, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, P.R. China.[4]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, Suzhou, 215123, P. R. China.
推荐引用方式(GB/T 7714)：
Ji Hu,Xin Qin,Zhen-Yuan Song,et al.Alpha-lipoic Acid suppresses P2X receptor activities and visceral hypersensitivity to colorectal distention in diabetic rats[J].SCIENTIFIC REPORTS.2017,7(1):3928.doi:10.1038/s41598-017-04283-7.
APA：
Ji Hu,Xin Qin,Zhen-Yuan Song,Pan-Pan Yang,Yu Feng...&Hong-Hong Zhang.(2017).Alpha-lipoic Acid suppresses P2X receptor activities and visceral hypersensitivity to colorectal distention in diabetic rats.SCIENTIFIC REPORTS,7,(1)
MLA：
Ji Hu,et al."Alpha-lipoic Acid suppresses P2X receptor activities and visceral hypersensitivity to colorectal distention in diabetic rats".SCIENTIFIC REPORTS 7..1(2017):3928
