机构：[a]Department of Neurology, the Second Affiliated Hospital of Soochow University,[b]Institute of Neuroscience, Soochow University, Suzhou[c]Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou[d]Suqian First Hospital, Suqian, China
Itch is a common symptom in patients with skin and systemic diseases, but the effective treatment is limited. Here, we evaluated the anti-itch effects of the botulinum toxin type A (BoNT/A) using acute and chronic dry skin itch mouse models, which were induced by compound 48/80, chloroquine, and a mixture of acetone-diethylether-water treatment, respectively. Pretreatment of intradermal BoNT/A exerted long-term inhibitory effects on compound 48/80-induced and chloroquine-induced acute itch on days 1, 3, 7, and 14, but not on day 21, in mice. Furthermore, a single injection of BoNT/A reduced the expression of the transient receptor potential cation channel, subfamily V, member 1 (TRPV1), and the transient receptor potential cation channel, subfamily A, member 1 (TRPA1) at both transcriptional and translational levels in the dorsal root ganglia (DRG) in mice. Pretreatment of BoNT/A also attenuated chronic itch induced by acetone-diethylether-water treatment and abolished the upregulation of TRPA1 in the DRG. Thus, it was suggested that downregulation of the expression of TRPA1 and TRPV1 in the DRG may contribute toward the long-term anti-itch effects of a single injection of BoNT/A in mice and BoNT/A treatment may serve as an alternative strategy for anti-itch therapy. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.
基金：
This work was supported by grants from the NSFC
(National Natural Science Foundation of China) to Tong
Liu (31371179, 81300968) and by a grant from the
Nature Science Foundation of Jiangsu province to Li-
Hua Chen (BK20140372). Tong Liu was also supported
by funding from Jiangsu Province (2015-JY-029). This
project is also supported by the Second Affiliated
Hospital of Soochow University Preponderant Clinic
Discipline Group Project Funding (XKQ2015002). This
project is also supported by A Project Funded by the
Priority Academic Program Development of Jiangsu
Higher Education Institutions.
第一作者机构：[a]Department of Neurology, the Second Affiliated Hospital of Soochow University,
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province 215004, China
推荐引用方式(GB/T 7714)：
Lei-Fang Cao,Meng Si,Ya Huang,et al.Long-term anti-itch effect of botulinum neurotoxin A is associated with downregulation of TRPV1 and TRPA1 in the dorsal root ganglia in mice[J].NEUROREPORT.2017,28(9):518-526.doi:10.1097/WNR.0000000000000779.
APA：
Lei-Fang Cao,Meng Si,Ya Huang,Li-Hua Chen,Xiao-Yan Peng...&Wei-Feng Luo.(2017).Long-term anti-itch effect of botulinum neurotoxin A is associated with downregulation of TRPV1 and TRPA1 in the dorsal root ganglia in mice.NEUROREPORT,28,(9)
MLA：
Lei-Fang Cao,et al."Long-term anti-itch effect of botulinum neurotoxin A is associated with downregulation of TRPV1 and TRPA1 in the dorsal root ganglia in mice".NEUROREPORT 28..9(2017):518-526
