机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China,[2]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu, P.R. China,[3]Department of Clinical Cancer Prevention, Division of Cancer Prevention and Population Sciences, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America,[4]The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, New York, United States of America
Polydatin (PD), a component isolated from Polygonum cuspidatum, has a number of biological functions. However, the antitumor activity of PD has been poorly investigated. In this study, the effect of PD on cell proliferation was evaluated by thiazolyl blue tetrazolium bromide assay. Cell cycle distribution and apoptosis were investigated by flow cytometry. The phosphorylation levels of panel of phosphor-kinases were detected by human phosphokinase arrays. The expression of several proteins associated with cell cycle and apoptosis were analyzed by Western blot analysis. Results showed that PD effectively inhibited the growth of MDA-MB-231 and MCF-7 breast cancer cell lines. Cell cycle analysis demonstrated that PD induced S-phase cell cycle arrest. Human phosphor-kinase arrays showed that the phosphorylation level of cAMP response element-bingding proteins(Creb) was down-regulated, and the results were further confirmed by Western blot analysis. Western blot analysis showed that the expression of protein of cyclin D1 decreased in a time-and dose-dependent manner. Results suggest that PD is a potential therapeutic natural compound.
基金：
The current study was supported by
grants from Suzhou Science and Technology
Program (SYS201345), Bureau of Public Health of
Jiangsu Province (H201413), pre-research project
from the second affiliated hospital of Soochow
university (SDFEYGJ1609) and the second
affiliated hospital of Soochow university clinical
discipline group project funding (XKQ 2015008).
第一作者机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China,[2]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu, P.R. China,
推荐引用方式(GB/T 7714)：
Chen Sijia,Tao Jialong,Zhong Fengyun,et al.Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell[J].PLOS ONE.2017,12(5):e0176501.doi:10.1371/journal.pone.0176501.
APA：
Chen, Sijia,Tao, Jialong,Zhong, Fengyun,Jiao, Yang,Xu, Jiaying...&Zhang, Yusong.(2017).Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell.PLOS ONE,12,(5)
MLA：
Chen, Sijia,et al."Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell".PLOS ONE 12..5(2017):e0176501
