机构：[1]Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.[2]Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, 100084, China.[3]Medprin Biotech GmbH, Gutleutstrae 163-167, Frankfurt am Main, 60327, Germany.[4]Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, 518055, China.
Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, some cell-cell interactions are limited by the existing hydrogel. To better mimic tumor microenvironment, self-assembled multicellular heterogeneous brain tumor fibers have been fabricated by a custom-made coaxial extrusion 3D bioprinting system, with high viability, proliferative activity and efficient tumor-stromal interactions. Therein, in order to further verify the sufficient interactions between tumor cells and stroma MSCs, CRE-LOXP switch gene system which contained GSCs transfected with "LOXP-STOP-LOXP-RFP" genes and MSCs transfected with "CRE recombinase" gene was used. Results showed that tumor-stroma cells interacted with each other and fused, the transcription of RFP was higher than that of 2D culture model and control group with cells mixed directly into alginate, respectively. RFP expression was observed only in the cell fibers but not in the control group under confocal microscope. In conclusion, coaxial 3D bioprinted multicellular self-assembled heterogeneous tumor tissue-like fibers provided preferable 3D models for studying tumor microenvironment in vitro, especially for tumor-stromal interactions.
基金：
1. Program of Medical Innovation Team
and Leading Talent of Jiangsu Province, China (No. LJ201150), 2. Science and Technology Plan Projects of
Jiangsu Province, China (No. BL2012048), 3. Clinical cutting-edge technology, social development projects
in Jiangsu Province (BE2016668), 4. Guangdong Innovative Research Team Program, China (No. 2011S055),
5. China Shenzhen Peacock Plan Project (No. KQTD201209), 6. The National High Technology Research and
Development Program of China (863 Program, No. 2015AA020303), 7. Graduate Research and Innovation
Program in Colleges and Universities of Jiangsu Province (No. KYLX16_0160).
第一作者机构：[1]Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
通讯作者：
通讯机构：[1]Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.[2]Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, 100084, China.[4]Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, 518055, China.
推荐引用方式(GB/T 7714)：
Xingliang Dai,Libiao Liu,Jia Ouyang,et al.Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers[J].SCIENTIFIC REPORTS.2017,7(1):1457.doi:10.1038/s41598-017-01581-y.
APA：
Xingliang Dai,Libiao Liu,Jia Ouyang,Xinda Li,Xinzhi Zhang...&Tao Xu.(2017).Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers.SCIENTIFIC REPORTS,7,(1)
MLA：
Xingliang Dai,et al."Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers".SCIENTIFIC REPORTS 7..1(2017):1457
