机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[2]Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[3]Molecular Medicine Center, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[4]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[5]Department of Interventional Radiology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[6]Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou 215004, China.
Background: Circulating cell-free DNA (ccf-DNA) in plasma may contain both specific and non-specific of tumor markers. The concentration and integrity of ccf-DNA may be clinical useful for detecting and predicting cancer progression. Methods: Plasma samples from 40 healthy controls and 73 patients with gastric cancers (two stage 0, 17 stage I, 11 stage II, 33 stage III, and 10 stage IV according to American Joint Committee on Cancer stage) were assessed respectively. qPCR targeting the Alu repeats was performed using two different sets of primers amplifying the long and short segments. DNA integrity was calculated as a ratio of the long to the short fragments of Alu repeats. Results: Plasma DNA concentration was significantly higher in patients with stage III and IV gastric cancers than in healthy controls (p = 0.028 and 0.029 respectively). The receiver operating characteristic (ROC) curve for discriminating patients with stage III and IV gastric cancers from healthy controls had an area under the curve (AUC) of 0.744 (95% CI, 0.64 to 0.85). Circulating cell-free DNA concentration increased within 21 days following surgery and dropped by 3 months after surgery. Conclusions: Concentration of ccf-DNA is a promising molecular marker for assessing gastric cancer progression.
基金：
This study was supported by the Jiangsu Province’s clinical medical science
and technology program (No. BL2013016), the Chinese National 863 Major
Grant (No. 2012AA020905), and the National Natural Science Foundation of
China (No. 81301267).
第一作者机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
通讯作者：
通讯机构：[4]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[6]Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou 215004, China.
推荐引用方式(GB/T 7714)：
Wang-Yang Pu,Rong Zhang,Li Xiao,et al.Prediction of cancer progression in a group of 73 gastric cancer patients by circulating cell-free DNA[J].BMC CANCER.2016,16(1):943.doi:10.1186/s12885-016-2977-7.
APA：
Wang-Yang Pu,Rong Zhang,Li Xiao,Yong-You Wu,Wei Gong...&Chun-Gen Xing.(2016).Prediction of cancer progression in a group of 73 gastric cancer patients by circulating cell-free DNA.BMC CANCER,16,(1)
MLA：
Wang-Yang Pu,et al."Prediction of cancer progression in a group of 73 gastric cancer patients by circulating cell-free DNA".BMC CANCER 16..1(2016):943
医学