机构：[a]Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, China[b]Department of Radiology, The Central Hospital of Wuhan, Wuhan, China[c]Institute of Radiotherapy & Oncology, Soochow University, Suzhou, China[d]Suzhou Key Laboratory for Radiation Oncology, Suzhou, China[e]Department of Radiology, Wuxi People’s Hospital, Wuxi, China[f]Magnetic Resonance Imaging Institute for Biomedical Research, Wayne State University, Detroit, MI, USA[g]Department of Neuroradiology and MRI, CHU Grenoble-IFR1, Grenoble, France
AIM: To evaluate oxygenation changes in rat subcutaneous C6 gliomas using blood-oxygen-level dependent (BOLD) functional magnetic resonance imaging (fMRI) combined with non-haemodynamic response function (non-HRF) analysis. MATERIALS AND METHODS: BOLD fMRI were performed during carbogen inhalation in 20 Wistar rats bearing gliomas. Statistical maps of spatial oxygenation changes were computed by a dedicated non-HRF analysis algorithm. Three types of regions of interest (ROIs) were defined: (1) maximum re-oxygenation zone (ROImax), (2) re-oxygenation zones that were less than the maximum re-oxygenation (ROInon-max), and (3) zones without significant re-oxygenation (ROInone). The values of percent BOLD signal change (PSC), percent enhancement (Delta SI), and significant re-oxygenation (T) were extracted from each ROI. Tumours were sectioned for histology using the fMRI scan orientation and were stained with haematoxylin and eosin and CD105. The number of microvessels (MVN) in each ROI was counted. Differences and correlations among the values for T, PSC, Delta SI, and MVN were determined. RESULTS: After carbogen inhalation, the PSC significantly increased in the ROImax areas (p<0.01) located in the tumour parenchyma. No changes occurred in any of the ROInone areas (20/20). Some changes occurred in a minority of the ROInon-max areas (3/60) corresponding to tumour necrosis. MVN and PSC (R = 0.59, p = 0.01) were significantly correlated in the ROImax areas. In the ROInon-max areas, MVN was significantly correlated with PSC (R = 0.55, p = 0.00) and Delta SI (R = 0.37, p = 0.00). CONCLUSIONS: Statistical maps obtained via BOLD fMRI with non-HRF analysis can assess the re-oxygenation of gliomas. (C) 2016 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.
基金：
This work was supported by the National Natural Science
Foundation of China (81271554), Jiangsu Provincial Special
Program of Clinical Medical Science (BL2014040), Suzhou
Science and Technology Development Program
(SZS201509), Call for Franco-Chinese projects in support of
decentralised cooperation by French Ministry of Foreign
Affairs in 2013, Open Project of Key Laboratory of Jiangsu
Provincial Molecular Nuclear Medicine (KF201308).
第一作者机构：[a]Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Radiology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China.
推荐引用方式(GB/T 7714)：
J. Xu,A. Chen,J. Xiao,et al.Evaluation of tumour vascular distribution and function using immunohistochemistry and BOLD fMRI with carbogen inhalation[J].CLINICAL RADIOLOGY.2016,71(12):1255-1262.doi:10.1016/j.crad.2016.04.012.
APA：
J. Xu,A. Chen,J. Xiao,Z. Jiang,Y. Tian...&J. Shen.(2016).Evaluation of tumour vascular distribution and function using immunohistochemistry and BOLD fMRI with carbogen inhalation.CLINICAL RADIOLOGY,71,(12)
MLA：
J. Xu,et al."Evaluation of tumour vascular distribution and function using immunohistochemistry and BOLD fMRI with carbogen inhalation".CLINICAL RADIOLOGY 71..12(2016):1255-1262
