机构：[1]Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China[2]Molecular Cardiology, Cleveland Clinic, Cleveland, U.S.A.[3]Thrombosis and Hemostasis Key Laboratory, Ministry of Education Engineering Center for Hematological Disease, Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Suzhou, China[4]Urology Department, the Second Affiliated Hospital of Soochow University, Suzhou, China
Atrial natriuretic peptide (ANP)-mediated natriuretic response is a well-established cardiac endocrine function. Corin is a transmembrane protease that activates ANP in the heart. Corin expression has been detected in non-cardiac tissues including the kidney. Here we examined corin, pro-ANP/ANP and natriuretic peptide receptor-A (NPR-A) expression in human renal segments. By immunostaining and in situ hybridization, we found similar corin, pro-ANP/ANP and NPR-A protein and mRNA expression in human renal segments. The expression was most abundant in the proximal convoluted tubules and the medullary connecting ducts. In the proximal tubules, corin protein was present in the apical membrane region underneath the brush border where the ANP-degrading protease neprilysin was abundant. These results suggest that corin-mediated pro-ANP activation may occur in renal segments and that locally produced ANP may act in an autocrine manner to regulate sodium and water reabsorption in situ. Our results also point to the proximal convoluted tubules as a major site for local ANP action. Such a renal corin/ANP autocrine mechanism may differ from the cardiac corin/ANP endocrine mechanism in regulating sodium homoeostasis under physiological and pathological conditions.
基金：
This work was supported by the National Natural Science Foundation
of China [grant numbers 81370718, 81570457 and
31500636]; the Ministry of Education of China [grant number
213016A]; and the Priority Academic Program Development of Jiangsu
Higher Education Institutes.
第一作者机构：[1]Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China
通讯作者：
通讯机构：[1]Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China[2]Molecular Cardiology, Cleveland Clinic, Cleveland, U.S.A.
推荐引用方式(GB/T 7714)：
Liang Dong,Hao Wang,Ningzheng Dong,et al.Localization of corin and atrial natriuretic peptide expression in human renal segments[J].CLINICAL SCIENCE.2016,130(18):1655-64.doi:10.1042/CS20160398.
APA：
Liang Dong,Hao Wang,Ningzheng Dong,Ce Zhang,Boxin Xue&Qingyu Wu.(2016).Localization of corin and atrial natriuretic peptide expression in human renal segments.CLINICAL SCIENCE,130,(18)
MLA：
Liang Dong,et al."Localization of corin and atrial natriuretic peptide expression in human renal segments".CLINICAL SCIENCE 130..18(2016):1655-64
