机构：[1]National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123 , P. R. China[2]School of Biology and Basic Medical Sciences, Soochow University Suzhou 215123 , P. R. China[3]National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123 , P. R. China[4]Department of Orthopedics, The Second Affi liated Hospital of Soochow University ,Suzhou 215000 , P. R. China[5]Department of Burns and Plastic Surgery, The Third Affi liated Hospital of Nantong University, Wuxi 214041 , P. R. China[6]Department of Biomedical Engineering, Tufts University, Medford , MA 02155 , USA
Functional vascularization is critical for the clinical regeneration of complex tissues such as kidney, liver, or bone. The immobilization or delivery of growth factors has been explored to improve vascularization capacity of tissue-engineered constructs; however, the use of growth factors has inherent problems such as the loss of signaling capability and the risk of complications including immunological responses and cancer. Here, a new method of preparing water-insoluble silk protein scaffolds with vascularization capacity using an all-aqueous process is reported. Acid is added temporally to tune the self-assembly of silk in the lyophilization process, resulting in water-insoluble scaffold formation directly. These biomaterials are mainly noncrystalline, offering improved cell proliferation than previously reported silk materials. These systems also have an appropriate softer mechanical property that could provide physical cues to promote cell differentiation into endothelial cells, and enhance neovascularization and tissue ingrowth in vivo without the addition of growth factors. Therefore, silk-based degradable scaffolds represent an exciting biomaterial option, with vascularization capacity for soft tissue engineering and regenerative medicine.
基金：
H.H., H.N., and S.L. contributed equally to this work. The authors
thank National Basic Research Program of China (973 Program,
2013CB934400), NSFC (21174097, 81272106, 51403144), and the
NIH (R01 DE017207, P41 EB002520). The authors also thank the
Priority Academic Program Development of Jiangsu Higher Education
Institutions (PAPD), the Excellent Youth Foundation of Jiangsu Province
(BK2012009), the Natural Science Foundation of Jiangsu Province (Grant
Nos. BK20140397 and BK20140401), and the Jiangsu Provincial Special
Program of Medical Science (BL2012004) for support of this work.
第一作者机构：[1]National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123 , P. R. China[2]School of Biology and Basic Medical Sciences, Soochow University Suzhou 215123 , P. R. China
通讯作者：
通讯机构：[3]National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123 , P. R. China[6]Department of Biomedical Engineering, Tufts University, Medford , MA 02155 , USA
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