机构：[a]National Engineering Laboratory for Modern Silk and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China[b]School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, People’s Republic of China[c]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, People’s Republic of China[d]Tai’an City Central Hospital, Taian 271000, People’s Republic of China[e]Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
Improving the controlled release of bioactive growth factors to regulate cell behavior and tissue regeneration remains a need in tissue engineering and regenerative medicine. Inorganic and polymeric nanoparticles have been extensively fabricated as bioactive biomaterials with enhanced biocompatibility and effective carriers of therapeutic agents, however, challenges remain such as the achievement of high loading capacity and sustained release, and the bioactivity preservation of growth factors. Here, a multilayered, silk coated hydroxyapatite (HA) nanocarrier with drug loading-release capacity superior to pure silk or HA nanoparticles was developed. Bone morphogenetic protein-2 (BMP-2) was bound to the silk coatings with a high binding efficiency of 99.6%, significantly higher than that in silk or the HA nanoparticles alone. The release of BMP-2 was sustained in vitro over a period of 21 days without burst release. Compared with BMP-2 loaded silk or HA particles, bone mesenchymal stem cells (BMSCs) showed improved proliferation and osteogenesis when cultured with the BMP-2 loaded composite nanocarriers. Therefore, these silk-HA composite nanoparticles are useful in designing bioactive nanocarrier systems with enhanced functions for bone tissue regeneration needs.
基金：
The authors thank the National Basic Research Program of
China (973 Program, 2013CB934400), NSFC (21174097, 51403144)
and the NIH (R01 DE017207, P41 EB002520). We also thank the
Priority Academic Program Development of Jiangsu Higher Education
Institutions (PAPD), the Excellent Youth Foundation of
Jiangsu Province (BK2012009), the Natural Science Foundation of
Jiangsu Province (Grant No. BK20140397 and BK20140401), and
the Key Natural Science Foundation of the Jiangsu Higher
Education Institutions of China (11KGA430002) for supporting
this work.
第一作者机构：[a]National Engineering Laboratory for Modern Silk and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China[b]School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[a]National Engineering Laboratory for Modern Silk and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China
推荐引用方式(GB/T 7714)：
Z. Z. Ding,Z. H. Fan,X. W. Huang,et al.Bioactive natural protein-hydroxyapatite nanocarriers for optimizing osteogenic differentiation of mesenchymal stem cells[J].JOURNAL OF MATERIALS CHEMISTRY B.2016,4(20):3555-3561.doi:10.1039/c6tb00509h.
APA：
Z. Z. Ding,Z. H. Fan,X. W. Huang,S. M. Bai,D. W. Song...&D. L. Kaplan.(2016).Bioactive natural protein-hydroxyapatite nanocarriers for optimizing osteogenic differentiation of mesenchymal stem cells.JOURNAL OF MATERIALS CHEMISTRY B,4,(20)
MLA：
Z. Z. Ding,et al."Bioactive natural protein-hydroxyapatite nanocarriers for optimizing osteogenic differentiation of mesenchymal stem cells".JOURNAL OF MATERIALS CHEMISTRY B 4..20(2016):3555-3561
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