机构：[a]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, PR China[b]Orthopedic Department, The Second Affiliated Hospital of Soochow University, Suzhou 215000, PR China[c]Cam-Su Genomic Resource Center, Soochow University, Suzhou 215123, PR China
Hematopoiesis is a complex process tightly controlled by sets of transcription factors in a context-dependent and stage-specific manner. Smad2/3 transcription factor plays a central role in differentiation and survival of erythroid cells. Here we report that follistatin-like 1 (FSTL1) treatment impairs hemin-induced erythroid differentiation and cell survival. FSTL1 differentially regulates transforming growth factor beta (TGF-beta) and bone morphogenetic protein (BMP) signaling. Blockade of Smad2/3 signaling with the ALK5/type I TGF-beta R kinase inhibitor, SB-525334, was efficacious for rescue of erythroid differentiation blockage and apoptosis. Reversely, activation of Smad1/5/8 signaling with BMP4 cannot rescue FSTL1-mediated erythroid differentiation blockage and apoptosis. Collectively, these data provide mechanistic insight into the regulation of erythropoiesis by FSTL1 signaling and lay a foundation for exploring FSTL1 signaling as a therapeutic target for anemia. (C) 2015 Elsevier Inc. All rights reserved.
基金：
This work was supported by National Natural Science Foundation
of China (NSFC-31401239 & NSFC-31301183), Jiangsu Province's
Natural Science Foundation for Colleges and Universities
(14KJB180021), Science and Technology project of Suzhou
(SYS201327), and Jiangsu Province's Key Discipline/Laboratory of
Medicine (XK201118).
第一作者机构：[b]Orthopedic Department, The Second Affiliated Hospital of Soochow University, Suzhou 215000, PR China
共同第一作者：
通讯作者：
通讯机构：[a]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, PR China
推荐引用方式(GB/T 7714)：
Jianping Wu,Yingying Dong,Xiaomei Teng,et al.Follistatin-like 1 attenuates differentiation and survival of erythroid cells through Smad2/3 signaling[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,466(4):711-6.doi:10.1016/j.bbrc.2015.09.044.
APA：
Jianping Wu,Yingying Dong,Xiaomei Teng,Maohua Cheng,Zhenya Shen&Weiqian Chen.(2015).Follistatin-like 1 attenuates differentiation and survival of erythroid cells through Smad2/3 signaling.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,466,(4)
MLA：
Jianping Wu,et al."Follistatin-like 1 attenuates differentiation and survival of erythroid cells through Smad2/3 signaling".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 466..4(2015):711-6
