机构：[a]Department of Neurosurgery, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China[b]Department of Pathology, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China
Wnt/beta-catenin signaling pathway is frequently dysregulated in human tumors and plays a critical role in tumorigenesis; however, the roles of microRNAs in mediating Wnt/b-catenin pathway are not well understood. Herein, we show that miR-30a-5p is activated by Wnt/beta-catenin pathway through direct binding of beta-catenin/TCF4 to two sites in the promoter region of miR-30a-5p. We also found that miR-30a-5p represses neural cell adhesion molecule (NCAM) expression by directly targeting two sites in the 3' untranslated region (3'-UTR) of NCAM mRNA. Moreover, Wnt/beta-catenin pathway represses NCAM expression in glioma cells, which depends on miR-30a-5p. Finally, we found that miR-30a-5p promotes glioma cell growth invasion by repressing NCAM. Our findings demonstrate a novel Wnt/beta-catenin-miR-30a-5p NCAM regulatory axis which plays important roles in controlling glioma cell invasion and tumorigenesis. (C) 2015 Elsevier Inc. All rights reserved.
基金：
This work was supported by
National Natural Science Foundation of China (81172400,
81272799, 81401500); Scientific and Technological Development
Program of Suzhou, China (SYS201477, SYSD2012090); University
Natural Science Foundation of Jiangsu, China (15KJB320011) and
Priority Academic Program Development of Jiangsu Higher Education
Institutions, China.
第一作者机构：[a]Department of Neurosurgery, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China
通讯作者：
通讯机构：[a]Department of Neurosurgery, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China
推荐引用方式(GB/T 7714)：
Zhongyong Wang,Xingliang Dai,Yanming Chen,et al.MiR-30a-5p is induced by Wnt/beta-catenin pathway and promotes glioma cell invasion by repressing NCAM[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,465(3):374-80.doi:10.1016/j.bbrc.2015.08.007.
APA：
Zhongyong Wang,Xingliang Dai,Yanming Chen,Chao Sun,Qing Zhu...&Qing Lan.(2015).MiR-30a-5p is induced by Wnt/beta-catenin pathway and promotes glioma cell invasion by repressing NCAM.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,465,(3)
MLA：
Zhongyong Wang,et al."MiR-30a-5p is induced by Wnt/beta-catenin pathway and promotes glioma cell invasion by repressing NCAM".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 465..3(2015):374-80
