机构：[1]Orthopedic Department, the First Affiliated Hospital of Soochow University, Suzhou, P.R. China,[2]Orthopedic Institute of Soochow University, Suzhou, P.R. China,[3]Orthopedic Department, the Second Affiliated Hospital of Soochow University, Suzhou, P.R. China,[4]Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, P.R. China,[5]State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P.R. China
Bone morphogenetic protein-2 (BMP-2) is a potent osteoinductive cytokine that plays a critical role in bone regeneration and repair. However, its distribution and side effects are major barriers to its success as therapeutic treatment. The improvement of therapy using collagen delivery matrices has been reported. To investigate a delivery system on posterolateral spinal fusion, both engineered human BMP-2 with a collagen binding domain (CBD-BMP-2) and collagen scaffolds were developed and their combination was implanted into Sprague-Dawley (SD) rats to study Lumbar 4-5 (L4-L5) posterolateral spine fusion. We divided SD rats into three groups, the sham group (G1, n = 20), the collagen scaffold-treated group (G2, n = 20) and the BMP-2-loaded collagen scaffolds group (G3, n = 20). 16 weeks after surgery, the spines of the rats were evaluated by X-radiographs, high-resolution micro-computed tomography (micro-CT), manual palpation and hematoxylin and eosin (H&E) staining. The results showed that spine L4-L5 fusions occurred in G2(40%) and G3(100%) group, while results from the sham group were inconsistent. Moreover, G3 had better results than G2, including higher fusion efficiency (X score, G2 = 2.4 +/- 0.163, G3 = 3.0 +/- 0, p<0.05), higher bone mineral density (BMD, G2: 0.3337 +/- 0.0025g/cm3, G3: 0.4353 +/- 0.0234g/cm3, p<0.05) and more bone trabecular formation. The results demonstrated that with site-specific collagen binding domain, a dose of BMP-2 as low as 0.02mg CBD-BMP-2/cm(3) collagen scaffold could enhance the posterolateral intertransverse process fusion in rats. It suggested that combination delivery could be an alternative in spine fusion with dramatically decreased side effects caused by high dose of BMP-2.
基金：
This work was supported by National Nature Science Foundation of China (81101369), the ‘‘Strategic Priority Research Program of the Chinese
Academy of Sciences’’ (Grant No. XDA01030000), SRF for ROCS of SEM (QS), PH.D. Program Foundation of SEM (20113201110013), Jiangsu Provincial Special
Program of Medical Science (BL2012004, BK2011264), and the State Key Laboratory Cultivation Base of Stem Cell and Bio-medical Materials.
第一作者机构：[1]Orthopedic Department, the First Affiliated Hospital of Soochow University, Suzhou, P.R. China,
共同第一作者：
通讯作者：
通讯机构：[1]Orthopedic Department, the First Affiliated Hospital of Soochow University, Suzhou, P.R. China,[5]State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P.R. China
推荐引用方式(GB/T 7714)：
Xinglong Han,Wen Zhang,Jun Gu,et al.Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats[J].PLOS ONE.2014,9(5):e98480.doi:10.1371/journal.pone.0098480.
APA：
Xinglong Han,Wen Zhang,Jun Gu,Huan Zhao,Li Ni...&Qin Shi.(2014).Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats.PLOS ONE,9,(5)
MLA：
Xinglong Han,et al."Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats".PLOS ONE 9..5(2014):e98480
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