机构：[1]Department of Neurology and Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, People’s Republic of China[2]The Institute of Neuroscience, Soochow University, 199 Ren-Ai Road, Suzhou 215123, Jiangsu, People’s Republic of China[3]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, People’s Republic of China[4]Department of Neurology, Suzhou Kowloon Hospital, 118 Wansheng Street, Suzhou 215021, Jiangsu, People’s Republic of China
Daphnetin (DAP), a coumarin derivative, has been reported to have multiple pharmacological actions including analgesia, antimalarial, anti-arthritic, and anti-pyretic properties. It is unclear whether DAP has neuroprotective effects on ischemic brain injury. In this study, we found that DAP treatment (i.c.v.) reduced the infarct volume at 24 h after ischemia/reperfusion injury and improved neurological behaviors in a middle cerebral artery occlusion mouse model. Moreover, we provided evidences that DAP had protective effects on infarct volume in neonate rats even it was administrated at 4 h after cerebral hypoxia/ischemia injury. To explore its neuroprotective mechanisms of DAP, we examined the protection of DAP on glutamate toxicity-induced cell death in hippocampal HT-22 cells. Our results demonstrated that DAP protected against glutamate toxicity in HT-22 cells in a concentration-dependent manner. Further, we found that DAP maintained the cellular levels of glutathione and superoxide dismutase activity, suggesting the anti-oxidatant activity of DAP. Since DAP has been used for the treatment of coagulation disorder and rheumatoid arthritis for long time with a safety profile, DAP will be a promising agent for the treatment of stroke.
基金：
This study was supported by Grants 30700245,
81071095, and 813111078 from the National Natural Science Foundation
of China, Suzhou science and technology development program
(SYS201232), the Priority Academic Program Development of Jiangsu
Higher Education Institutions of China, Jiangsu Key Laboratory of
Translational Research (BM2013003), and Jiangsu Province’s Key
Provincial Talents Program
第一作者机构：[1]Department of Neurology and Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, People’s Republic of China[2]The Institute of Neuroscience, Soochow University, 199 Ren-Ai Road, Suzhou 215123, Jiangsu, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology and Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, People’s Republic of China
推荐引用方式(GB/T 7714)：
Gang Du,Hui Tu,Xiaojing Li,et al.Daphnetin, a Natural Coumarin Derivative, Provides the Neuroprotection Against Glutamate-Induced Toxicity in HT22 Cells and Ischemic Brain Injury[J].NEUROCHEMICAL RESEARCH.2014,39(2):269-75.doi:10.1007/s11064-013-1218-6.
APA：
Gang Du,Hui Tu,Xiaojing Li,Aijie Pei,Jing Chen...&Heqing Zhao.(2014).Daphnetin, a Natural Coumarin Derivative, Provides the Neuroprotection Against Glutamate-Induced Toxicity in HT22 Cells and Ischemic Brain Injury.NEUROCHEMICAL RESEARCH,39,(2)
MLA：
Gang Du,et al."Daphnetin, a Natural Coumarin Derivative, Provides the Neuroprotection Against Glutamate-Induced Toxicity in HT22 Cells and Ischemic Brain Injury".NEUROCHEMICAL RESEARCH 39..2(2014):269-75
