机构：[1]Department of Gastroenterology, The Second Affiliated Hospital of Soochow University[2]Department of Hepatology, The First Affiliated Hospital of Soochow University, Soochow[3]Department of Gastroenterology, The Second People’s Hospital of Huai’an, Huai’an[4]Experimental Center, The Fifth People’s Hospital of Soochow[5]Experimental Center, The Second Affiliated Hospital of Soochow University, Soochow, People’s Republic of China[6]Department of Pathology, The Second Affiliated Hospital of Soochow University, Soochow, People’s Republic of China
Objectives: The objective of this study was to investigate the effects of rat umbilical cord mesenchymal stem cells (UCMSCs) from Wharton's jelly on dibutyltin dichloride (DBTC) induced chronic pancreatitis (CP) and subsequent pancreatic fibrosis in rats. Methods: A rat model of CP induced by DBTC was used. Male Sprague-Dawley rats were randomly divided into 4 groups: the control, DBTC, DBTC + UCMSCs, and control + UCMSC groups. Umbilical cord mesenchymal stem cells were administered intravenously on day 5 after the administration of DBTC. On days 14 and 28, the rats were evaluated morphologically and biochemically. The expression levels of inflammatory cytokines and chemokines in the pancreatic tissues of different groups were evaluated using quantitative real-time polymerase chain reaction. The activation of pancreatic stellate cells was estimated by immunochemistry and Western blot analysis of a-smooth muscle actin. Results: Umbilical cord mesenchymal stem cells were detected in inflamed pancreatic tissues. Umbilical cord mesenchymal stem cell treatment improved the histological scores and alleviated the fibrosis of pancreas samples, The expression of cytokines in the DBTC + UCMSC group was significantly lower than that in the DBTC group. Also, pancreatic stellate cell activation was inhibited by UCMSC treatment. Conclusions: Xenogeneic transplantation of UCMSCs is a novel approach for the treatment of CP and subsequent fibrosis. Umbilical cord mesenchymal stem cells may be a promising therapeutic intervention for human CP in the future.
基金：
This work was supported by a grant from the Health Bureau of Suzhou (project no. SYZ201128).
第一作者机构：[1]Department of Gastroenterology, The Second Affiliated Hospital of Soochow University
共同第一作者：
通讯作者：
通讯机构：[*]Department of Gastroenterology, The Second Affiliated Hospital of Soochow University, San-Xiang Rd 1055, Suzhou City, JiangSu Province, 215004 People’s Republic of China
推荐引用方式(GB/T 7714)：
Chun-Hua Zhou ,Mei-Lin Li ,Ai-Lan Qin,et al.Reduction of Fibrosis in Dibutyltin Dichloride-Induced Chronic Pancreatitis Using Rat Umbilical Mesenchymal Stem Cells From Wharton's Jelly[J].PANCREAS.2013,42(8):1291-302.doi:10.1097/MPA.0b013e318296924e.
APA：
Chun-Hua Zhou,,Mei-Lin Li,,Ai-Lan Qin,Sheng-Xiang Lv,Wen-Tang...&Shao-Feng Wang.(2013).Reduction of Fibrosis in Dibutyltin Dichloride-Induced Chronic Pancreatitis Using Rat Umbilical Mesenchymal Stem Cells From Wharton's Jelly.PANCREAS,42,(8)
MLA：
Chun-Hua Zhou,,et al."Reduction of Fibrosis in Dibutyltin Dichloride-Induced Chronic Pancreatitis Using Rat Umbilical Mesenchymal Stem Cells From Wharton's Jelly".PANCREAS 42..8(2013):1291-302
