机构：[1]Laboratory of Cancer Molecular Genetics, Medical College of Soochow University[2]Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University, Suzhou[3]Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University, Suzhou[4]Department of Otorhinolaryngology-Head and Neck Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou[5]The Institute for Chemical Carcinogenesis, The State Key Lab of Respiratory Disease, Guangzhou Medical University, Guangzhou, China
Nasopharyngeal carcinoma is treated with radiotherapy and other modalities, but there is little information on individual genetic factors to help predict and improve patient outcomes. Single-nucleotide polymorphisms (SNP) in mature microRNA (miRNA) sequences have the potential to exert broad impact as miRNAs target many mRNAs. The aim of this study was to evaluate the effects of SNPs in mature miRNA sequences on clinical outcome in patients with nasopharyngeal carcinoma receiving radiotherapy. In particular, we analyzed associations between seven SNPs and nasopharyngeal carcinoma locoregional recurrence (LRR) in 837 patients from eastern China, validating the findings in an additional 828 patients from southern China. We found that miR-608 rs4919510C>G exhibited a consistent association with LRR in the discovery set [HR, 2.05; 95% confidence interval (CI), 1.35-3.21], the validation set (HR, 2.24; 95% CI, 1.45-3.38), and the combined dataset (HR, 2.08; 95% CI, 1.41-3.26). Biochemical investigations showed that rs4919510C>G affects expression of miR-608 target genes along with nasopharyngeal carcinoma cell growth after irradiation in vivo and in vitro. Notably, X-ray radiation induced more chromatid breaks in lymphocyte cells from rs4919510CC carriers than in those from subjects with other genotypes (P = 0.0024). Our findings reveal rs4919510C> G in miR-608 as a simple marker to predict LRR in patients with radiotherapy-treated nasopharyngeal carcinoma. (C) 2013 AACR.
基金：
This study was supported by the National Scientific Foundation of China
grants 81001278, 81171895, and 81072366; a project funded by the Priority
Academic Program Development of Jiangsu Higher Education Institutions,
Jiangsu Provincial Natural Science Foundation (no. BK2011297); Jiangsu Province
Science and Technology Support Program (no. BE2012648); the Scientific
Research Foundation for the Returned Overseas Chinese Scholars, State Education
Ministry (no. 20101561), Suzhou Key Laboratory for Molecular Cancer
Genetics (no. SZS201209); and Zhejiang Provincial Natural Science Foundation
of China (Y2100449 and Y2110410).
第一作者机构：[1]Laboratory of Cancer Molecular Genetics, Medical College of Soochow University
共同第一作者：
通讯作者：
通讯机构：[*]Medical College of Soochow University, No.199 Ren-ai Rd., Suzhou 215123, China.
推荐引用方式(GB/T 7714)：
Jian Zheng,Jieqiong Deng,Mang Xiao,et al.A Sequence Polymorphism in miR-608 Predicts Recurrence after Radiotherapy for Nasopharyngeal Carcinoma[J].CANCER RESEARCH.2013,73(16):5151-62.doi:10.1158/0008-5472.CAN-13-0395.
APA：
Jian Zheng,Jieqiong Deng,Mang Xiao,Lei Yang,Liyuan Zhang...&Yifeng Zhou.(2013).A Sequence Polymorphism in miR-608 Predicts Recurrence after Radiotherapy for Nasopharyngeal Carcinoma.CANCER RESEARCH,73,(16)
MLA：
Jian Zheng,et al."A Sequence Polymorphism in miR-608 Predicts Recurrence after Radiotherapy for Nasopharyngeal Carcinoma".CANCER RESEARCH 73..16(2013):5151-62
医学