机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmacy, Soochow University, Suzhou 215004, China
Iron overload has recently been connected with bone mineral density in osteoporosis. However, to date, the effect of iron overload on osteoblasts remains poorly understood. The purpose of this study is to examine osteoblast biological activity under iron overload. The osteoblast cells (hFOB1.19) were cultured in a medium supplemented with different concentrations (50, 100, and 200 mu M) of ferric ammonium citrate as a donor of ferric ion. Intracellular iron was measured with a confocal laser scanning microscope. Reactive oxygen species (ROS) were detected by 2,7-dichlorofluorescin diacetate fluorophotometry. Osteoblast biological activities were evaluated by measuring the activity of alkaline phosphatase (ALP) and mineralization function. Results indicated that iron overload could consequently increase intracellular iron concentration and intracellular ROS levels in a concentration-dependent manner. Additionally, ALP activity was suppressed, and a decline in the number of mineralized nodules was observed in in vitro cultured osteoblast cells. According to these results, it seems that iron overload probably inhibits osteoblast function through higher oxidative stress following increased intracellular iron concentrations.
基金：
This work was partially supported by the National
Natural Science Foundation of China (no. 81273090), Jiangsu
provincial grant (no. BK2012608), and science and technology projects
of Suzhou (no. 510303).
第一作者机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
推荐引用方式(GB/T 7714)：
Yin-Feng He,Yong Ma,Chao Gao,et al.Iron Overload Inhibits Osteoblast Biological Activity Through Oxidative Stress[J].BIOLOGICAL TRACE ELEMENT RESEARCH.2013,152(2):292-6.doi:10.1007/s12011-013-9605-z.
APA：
Yin-Feng He,Yong Ma,Chao Gao,Guo-yang Zhao,Lin-Lin Zhang...&You-Jia Xu.(2013).Iron Overload Inhibits Osteoblast Biological Activity Through Oxidative Stress.BIOLOGICAL TRACE ELEMENT RESEARCH,152,(2)
MLA：
Yin-Feng He,et al."Iron Overload Inhibits Osteoblast Biological Activity Through Oxidative Stress".BIOLOGICAL TRACE ELEMENT RESEARCH 152..2(2013):292-6
