In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson's Disease Rats Using Magnetic Resonance Imaging
机构：[1]Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, China[2]Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[3]Department of Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China[4]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, China[5]IPS, Sherbrooke University Medical School, Quebec, Canada[6]The Medical College of Soochow University, Suzhou 215123, China
The effectiveness of Resovist-labeled bone marrow stem cells (BMSCs) was evaluated in vivo following their cerebral transplantation in a model of Parkinson's disease (PD) in rats using MRI, and the MRI findings were further compared with the behavior and histopathological manifestations of these rats. Forty PD rats were randomly assigned into five groups according to the cell doses injected into the rat brain site: control group (normal saline injection) and groups injected with 1x10(5), 1.5x10(5), 2x10(5), and 2.5x10(5) BMSCs. Gradient echo T2-weighted images were obtained immediately after cell transplantation and repeatedly taken 1, 4, 8, and 12 week(s) after cell transplantation. The rotational behavior of the animals was observed before and 1, 4, and 8 week(s) after transplantation. The rats were killed after the last MRI scanning, the brain tissues were analyzed by histopathology techniques, and RNAs were extracted for the expression analysis of selected genes using RT-PCR. One week following cell transplantation, all injected sites showed well-defined hypointense areas on MR images, with the most significant effect observed in rats injected with 2x10(5) BMSCs. These MR findings in PD rats lasted up to 12 weeks. The effectiveness of BMSC transplantation revealed by MRI was well confirmed by the behavioral and histopathological observations as well as indirectly supported by gene expression analyses. With the use of SPIO labeling, MRI techniques provided a dynamic evaluation of the spatial and temporal changes following cell transplantation and allowed the association analysis among the imaging, functions, and gene expression analysis in rats. These data also suggest the therapeutic potential of transplanted BMSCs. It is reasonable to speculate that the use of MRI in in vivo evaluation of the effect and fate of transplanted cells in various disease models will be beneficial to developing new strategies of cell-based gene therapy.
基金：
This research was supported by the Natural science fund for colleges and universities in
Jiangsu Province (no. 06KJB320097).
第一作者机构：[1]Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, China[6]The Medical College of Soochow University, Suzhou 215123, China
推荐引用方式(GB/T 7714)：
Jing Guo,Jun-Kang Shen,Lan Wang,et al.In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson's Disease Rats Using Magnetic Resonance Imaging[J].APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY.2011,163(5):636-48.doi:10.1007/s12010-010-9069-y.
APA：
Jing Guo,Jun-Kang Shen,Lan Wang,Li Xiao,Rong-Jun Zhang...&Kai Li.(2011).In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson's Disease Rats Using Magnetic Resonance Imaging.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,163,(5)
MLA：
Jing Guo,et al."In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson's Disease Rats Using Magnetic Resonance Imaging".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 163..5(2011):636-48
