机构：[1]College of Pharmacy, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, 830054, China.[2]Xinjiang Institute of Materia Medica, No. 140 Xinhua South Road, Urumqi, China.[3]Department of Experimental Animal Center, Xuanwu Hospital of Capital Medical University, No. 45 Changchun Street, Beijing, China.首都医科大学宣武医院
We previously demonstrated the cardio-protection mediated by the total flavonoid extracted from Dracocephalum moldavica L. (TFDM) following myocardial ischemia reperfusion injury (MIRI). The present study assessed the presence and mechanism of TFDM-related cardio-protection on MIRI-induced apoptosis in vivo. Male Sprague-Dawley rats experienced 45-min ischemia with 12 h of reperfusion. Rats pretreated with TFDM (3, 10 or 30 mg/kg/day) were compared with Sham (no MIRI and no TFDM), MIRI (no TFDM), and Positive (trapidil tablets, 13.5 mg/kg/day) groups. In MIRI-treated rats, high dose-TFDM (H-TFDM) pre-treatment with apparently reduced release of LDH, CK-MB and MDA, enhanced the concentration of SOD in plasma, and greatly reduced the infarct size, apoptotic index and mitochondrial injury. H-TFDM pretreatment markedly promoted the phosphorylation of PI3K, Akt, GSK-3 beta and ERK1/2 in comparison with the MIRI model group. Western blot analysis after reperfusion also showed that H-TFDM decreased release of Bax, cleaved caspase-3, caspase-7 and caspase-9, and increased expression of BcI-2 as evident by the higher BcI-2/Bax ratio. TFDM cardio-protection was influenced by LY294002 (PI3K inhibitor) and PD98059 (ERK1/2 inhibitor). Taken together, these results provide convincing evidence of the benefit of TFDM pretreatment due to inhibited myocardial apoptosis as mediated by the Pl3K/Akt/GSK-3 beta and ERK1/2 signaling pathways.
基金：
The current research was sponsored by the National Natural Science Foundation of China (H0203), Autonomous
Region High Technology Research and Development Program of China (201517109) and Plan for Supporting
Xinjiang through Science and Technology in Xinjiang Uygur Autonomous Region (201591149).
第一作者机构：[1]College of Pharmacy, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, 830054, China.[2]Xinjiang Institute of Materia Medica, No. 140 Xinhua South Road, Urumqi, China.[3]Department of Experimental Animal Center, Xuanwu Hospital of Capital Medical University, No. 45 Changchun Street, Beijing, China.
通讯作者：
通讯机构：[1]College of Pharmacy, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, 830054, China.[2]Xinjiang Institute of Materia Medica, No. 140 Xinhua South Road, Urumqi, China.[3]Department of Experimental Animal Center, Xuanwu Hospital of Capital Medical University, No. 45 Changchun Street, Beijing, China.
推荐引用方式(GB/T 7714)：
Cheng Zeng,Wen Jiang,Xiaoyi Yang,et al.Pretreatment with Total Flavonoid Extract from Dracocephalum Moldavic L. Attenuates Ischemia Reperfusion-induced Apoptosis[J].SCIENTIFIC REPORTS.2018,8(1):17491.doi:10.1038/s41598-018-35726-4.
APA：
Cheng Zeng,Wen Jiang,Xiaoyi Yang,Chenghui He,Wen Wang&Jianguo Xing.(2018).Pretreatment with Total Flavonoid Extract from Dracocephalum Moldavic L. Attenuates Ischemia Reperfusion-induced Apoptosis.SCIENTIFIC REPORTS,8,(1)
MLA：
Cheng Zeng,et al."Pretreatment with Total Flavonoid Extract from Dracocephalum Moldavic L. Attenuates Ischemia Reperfusion-induced Apoptosis".SCIENTIFIC REPORTS 8..1(2018):17491
