机构：[a]China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing, China中美神经科学研究所首都医科大学宣武医院[b]Department of Neurology, Beijing Luhe Hospital, Capital Medical University, Beijing, China[c]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China神经外科首都医科大学宣武医院[d]Department of Neurosurgery, Stanford University, Stanford, CA, United States
Long noncoding RNAs (lncRNAs) have a variety of biological functions and play key roles in many diseases. However, the knowledge of lncRNA function during a stroke is limited. We analyzed the expression profiles of lncRNAs in the brain ischemic region of mice after a 45 min middle cerebral artery occlusion (MCAO) with a 48 h reperfusion. Gene ontology and pathway analysis were used to elucidate the potential functions of the differentially expressed mRNAs. A total of 255 lncRNAs (217 up-regulated and 38 down-regulated) and 894 mRNAs (870 up-regulated and 24 down-regulated) showed significantly altered expression in the ischemic brain compared to the sham controls (fold change >> > 2, P << 0.05). The gene ontology terms were mainly associated with neutrophil chemotaxis, positive regulation of inflammatory response, cell cycle, positive regulation of apoptotic process, and apoptotic process. The pathway analysis indicated that the mRNAs were mainly associated with inflammatory pathways. Additionally, the interactions between the differentially expressed lncRNAs and mRNAs are revealed by a dynamic lncRNA-mRNA network. Our findings provide an overview of aberrantly expressed lncRNAs in stroke and further broaden the understanding of stroke pathogenesis.
基金：
the National Natural Science Foundation of China(81701154),
Beijing Natural Science Foundation (7172109) and R01NS06413606
第一作者机构：[a]China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Stanford University School of Medicine, MSLS Bldg., P306, 1201 Welch Rd., Rm. P306, Stanford, CA 94305-5327, United States.[*2]Department of Neurology, China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Cuiying Liu,Jian Yang,Chencheng Zhang,et al.Analysis of long non-coding RNA expression profiles following focal cerebral ischemia in mice[J].NEUROSCIENCE LETTERS.2018,665:123-129.doi:10.1016/j.neulet.2017.11.058.
APA：
Cuiying Liu,Jian Yang,Chencheng Zhang,Menglei Liu,Xiaokun Geng...&Heng Zhao.(2018).Analysis of long non-coding RNA expression profiles following focal cerebral ischemia in mice.NEUROSCIENCE LETTERS,665,
MLA：
Cuiying Liu,et al."Analysis of long non-coding RNA expression profiles following focal cerebral ischemia in mice".NEUROSCIENCE LETTERS 665.(2018):123-129
