Objective MicroRNAs (miRs) are attractive molecules to be considered as one of the blood-based biomarkers for neurodegenerative disorders such as Alzheimer's disease (AD). The goal of this study was to explore their potential value as biomarkers for the diagnosis of AD. Methods The expression levels of exosomal miR-135a, -193b, and -384 in the serum from mild cognitive impairment (MCI), dementia of Alzheimer-type (DAT), Parkinson's disease with dementia (PDD), and vascular dementia (VaD) patients were measured with a real-time quantitative reverse transcriptase PCR (qRT-PCR) method. Results Both serum exosome miR-135a and miR-384 were up-regulated while miR-193b was down-regulated in serum of AD patients compared with that of normal controls. Exosome miR-384 was the best among the three miRs to discriminate AD, VaD, and PDD. Using the cut-off value could better interpret these laboratory test results than reference intervals in the AD diagnosis. ROC curve showed that the combination of miR-135a, -193b, and -384 was proved to be better than a particular one for early AD diagnosis. Conclusion Our results indicated that the exosomal miRs in the serum were not only potential biomarker of AD early diagnosis, but might also provide novel insights into the screen and prevention of the disease.
基金：
the National Key Research and Development Program of China [2016YFC1306300];
the National Natural Science Foundation of China [No. 81401734];
the National Research Foundation for the Doctoral Program of Higher Education of China [No. 20121107110001];
the National Natural Science Foundation of China [No. 81472007].
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中科院(CAS)分区：
出版当年[2017]版：
大类|3 区环境科学与生态学
小类|3 区环境科学3 区公共卫生、环境卫生与职业卫生
最新[2025]版：
大类|4 区医学
小类|4 区环境科学4 区公共卫生、环境卫生与职业卫生
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出版当年[2016]版：
Q2ENVIRONMENTAL SCIENCESQ2PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
最新[2023]版：
Q2ENVIRONMENTAL SCIENCESQ2PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
