MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway
机构：[a]Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun[b]Department of Nephrology, Xuanwu Hospital, Capital Medical University, Beijing肾科首都医科大学宣武医院[c]Department of Vascular Surgery, Jilin Provincial People’s Hospital, Changchun[d]Department of Urology, China-Japan Union Hospital of Jilin University, Changchun, P.R. China
Background/Aims: The role of the RhoA/Rho kinase signaling pathway in diabetes mellitus-induced erectile dysfunction has been partially understood. Methods: In the present study, we explored the changes of the RhoA/Rho associated kinase (ROCK) signaling pathway in diabetic erectile dysfunction in vivo and the effects of microRNA-141 on the RhoA/ROCK signaling pathway in vitro. Results: The mRNA and protein expressions of RhoA and ROCK2 were significantly increased while the expression of microRNA-141 was decreased in the penile cavernous smooth muscle cells of rats with diabetic erectile dysfunction. Moreover, increased expression of microRNA-141, decreased expressions of RhoA and ROCK2 (mRNA and protein), accelerated cell proliferation rate and reduced cell apoptosis were found in the microRNA-141 mimics group and the siRNA-Rho group. The microRNA-141 expression in the microRNA-141 inhibitors + siRNA-Rho group was significantly decreased. microRNA-141 specifically bound to Rho-3'-UTR and down-regulated the expression of Rho gene at the post transcriptional level. Conclusion: Decreased expression of miR-141 is associated with up-regulation of RhoA and ROCK2 in the RhoA/ROCK signaling pathway in rats with diabetic erectile dysfunction. miR-141 inhibits the growth of penile cavernous smooth muscle cells associated with down regulation of the RhoA/ROCK signaling pathway in vitro. (C) 2018 The Author(s) Published by S. Karger AG, Basel
第一作者机构：[a]Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun
通讯作者：
通讯机构：[*1]Department of Urology, China-Japan Union Hospital of Jilin University,No. 126, Xiantai Street, 130033 Changchun, Jilin Province, (P.R. China)
推荐引用方式(GB/T 7714)：
Ying Zhang,Linpei Jia,Wei Ji,et al.MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2018,48(1):348-360.doi:10.1159/000491741.
APA：
Ying Zhang,Linpei Jia,Wei Ji&Hai Li.(2018).MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,48,(1)
MLA：
Ying Zhang,et al."MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 48..1(2018):348-360
