机构：[1]China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.中美神经科学研究所首都医科大学宣武医院[2]Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China.[3]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.神经外科首都医科大学宣武医院[4]Department of Radiology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.放射科首都医科大学宣武医院[5]Department of Anesthesiology, XuanWu Hospital, Capital Medical University, Beijing 100053, China.麻醉手术科首都医科大学宣武医院[6]Department of Laboratory Animal Science, Capital Medical University, Beijing, China.[7]Department of Neurological Surgery, the Ohio State University Wexner Medical Center, Columbus, OH, USA.[8]Department of Neurological Surgery, Wayne State University School of Medicine, Detroit, MI, USA.
To further investigate and improve upon current stroke models in nonhuman primates, infarct size, neurologic function and survival were evaluated in two endovascular ischemic models in sixteen rhesus monkeys. The first method utilized a micro-catheter or an inflatable balloon to occlude the M1 segment in six monkeys. In the second model, an autologous clot was injected via a micro-catheter into the M1 segment in ten monkeys. MRI scanning was performed on all monkeys both at baseline and 3 hours after the onset of ischemia. Spetzler neurologic functions were assessed post-operatively, and selective perfusion deficits were confirmed by DSA and MRI in all monkeys. Animals undergoing micro-catheter or balloon occlusion demonstrated more profound hemiparesis, larger infarct sizes, lower Spetzler neurologic scores and increased mortality compared to the thrombus occlusion group. In animals injected with the clot, there was no evidence of dissolution, and the thrombus was either near the injection site ( M1) or flushed into the superior division of the MCA (M2). All animals survived the M2 occlusion. M1 occlusion with thrombus generated 50% mortality. This study highlighted clinically important differences in these two models, providing a platform for further study of a translational thromboembolic model of acute ischemic stroke.
基金：
National Natural Science Foundation of China for Outstanding Youth(81325007);
National Natural Science Foundation of China (81500997);
Chang Jiang Scholars Program (#T2014251) from the Chinese Ministry of Education.
National Key Basic Research Program (973 Program) of China (2011CB707804);
Beijing Municipal Science and Technology Project, No. 2121100005312016
第一作者机构：[1]China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.[2]Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China.
通讯作者：
通讯机构：[1]China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.[2]Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China.[3]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.
推荐引用方式(GB/T 7714)：
Di Wu,Jian Chen,Bincheng Wang,et al.Endovascular ischemic stroke models of adult rhesus monkeys: a comparison of two endovascular methods[J].SCIENTIFIC REPORTS.2016,6:doi:10.1038/srep31608.
APA：
Di Wu,Jian Chen,Bincheng Wang,Mo Zhang,Jingfei Shi...&Xunming Ji.(2016).Endovascular ischemic stroke models of adult rhesus monkeys: a comparison of two endovascular methods.SCIENTIFIC REPORTS,6,
MLA：
Di Wu,et al."Endovascular ischemic stroke models of adult rhesus monkeys: a comparison of two endovascular methods".SCIENTIFIC REPORTS 6.(2016)
