机构：[a]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China老年医学科首都医科大学宣武医院[b]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[c]Center of Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100069, China[d]Department of Neurobiology, Beijing Key Laboratory of Major Brain Disorders, Beijing Institute of Brain Disorders, Capital Medical University, Beijing 100069, China
Human neural stem cells (NSCs) hold great promise for research and therapy in neural diseases. Many studies have shown direct induction of NSCs from human fibroblasts, which require an invasive skin biopsy and a prolonged period of expansion in cell culture prior to use. Peripheral blood (PB) is routinely used in medical diagnoses, and represents a noninvasive and easily accessible source of cells. Here we show direct derivation of NSCs from adult human PB mononuclear cells (PB-MNCs) by employing episomal vectors for transgene delivery. These induced NSCs (iNSCs) can expand more than 60 passages, can exhibit NSC morphology, gene expression, differentiation potential, and self-renewing capability and can give rise to multiple functional neural subtypes and glial cells in vitro. Furthermore, the iNSCs carry a specific regional identity and have electrophysiological activity upon differentiation. Our findings provide an easily accessible approach for generating human iNSCs which will facilitate disease modeling, drug screening, and possibly regenerative medicine. (C) 2016 The Authors. Published by Elsevier B.V.
基金：
the National Basic Research Program of China (2011CB965103 and 2012CBA01307),
National Natural Science Foundation of China (81422014, 81561138004, and 31340075),
Beijing Municipal Natural Science Foundation (5142005),
Technology Foundation for Selected Overseas Chinese, MOHRSS (2014).
第一作者机构：[a]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China[b]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[c]Center of Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100069, China
通讯作者：
通讯机构：[a]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China[*1]Cell Therapy Center, XuanwuHospital, CapitalMedical University, Beijing 100053, China.
推荐引用方式(GB/T 7714)：
Xihe Tang,Shuyan Wang,Yunfei Bai,et al.Conversion of adult human peripheral blood mononuclear cells into induced neural stem cell by using episomal vectors[J].STEM CELL RESEARCH.2016,16(2):236-242.doi:10.1016/j.scr.2016.01.016.
APA：
Xihe Tang,Shuyan Wang,Yunfei Bai,Jianyu Wu,Linlin Fu...&Zhiguo Chen.(2016).Conversion of adult human peripheral blood mononuclear cells into induced neural stem cell by using episomal vectors.STEM CELL RESEARCH,16,(2)
MLA：
Xihe Tang,et al."Conversion of adult human peripheral blood mononuclear cells into induced neural stem cell by using episomal vectors".STEM CELL RESEARCH 16..2(2016):236-242
