Background: The activity of eukaryotic DNA polymerase delta (Pol delta) plays an essential role in genome stability through its effects on DNA replication and repair. The p125 catalytic subunit of Pol d is encoded by POLD1 gene in human cells. To clarify biological functions of POLD1, we investigated the effects of POLD1 overexpression or downregulation on cell proliferation, cell cycle progression, DNA synthesis and oxidative DNA damage induced by H2O2. Methods: HEK293 cells were transfected with POLD1 expression plasmid or shRNA, cell proliferation, cell cycle progression, and DNA synthesis in HEK293 cells were analyzed. Results: HEK293 cells were transfected with POLD1 expression plasmid or shRNA. POLD1 downregulation by shRNA suppressed cell proliferation, cell cycle progression, and DNA synthesis in HEK293 cells. However, POLD1 overexpression had no significant effects on these processes. Finally, comet assay showed that POLD1 downregulation led to increased DNA damage. Conclusions: Our results suggest that human POLD1 plays important role in the regulation of cell cycle progression and DNA damage repair.
基金：
the National Natural Science Foundation of China (grant no.81271924 and no. 81472007).
第一作者机构：[1]Department of Clinical Laboratory, Xuanwu Hospital Capital Medical University, No.45 Changchun Street, Xicheng District, Beijing, 100053, China
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, Xuanwu Hospital Capital Medical University, No.45 Changchun Street, Xicheng District, Beijing, 100053, China
推荐引用方式(GB/T 7714)：
Jing Song,Ping Hong,Chengeng Liu,et al.Human POLD1 modulates cell cycle progression and DNA damage repair[J].BMC BIOCHEMISTRY.2015,16(1):14.doi:10.1186/s12858-015-0044-7.
APA：
Jing Song,Ping Hong,Chengeng Liu,Yueqi Zhang,Jinling Wang&Peichang Wang.(2015).Human POLD1 modulates cell cycle progression and DNA damage repair.BMC BIOCHEMISTRY,16,(1)
MLA：
Jing Song,et al."Human POLD1 modulates cell cycle progression and DNA damage repair".BMC BIOCHEMISTRY 16..1(2015):14
