机构：[1]Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China[2]Beijing Center of Neural Regeneration and Repair, Capital Medical University, Beijing, China[3]Department of Neurobiology, Capital Medical University, Beijing, China[4]Beijing Key Laboratory of Major Brain Disorders, Capital Medical University, Beijing, China[5]Beijing Municipal Key Laboratory for Neural Regeneration and Repairing, Capital Medical University, Beijing, China[6]Department of Cell Biology, Xuanwu Hospital of Capital Medical University, Beijing, China首都医科大学宣武医院
In the present study, induced pluripotent stem cells (iPSCs), induced neural stem cells (iNSCs), mesenchymal stem cells (MSCs) and an immortalized cell line (RMNE6), representing different characteristics of stem cells, were transplanted into normal and/or injured brain areas of rodent stroke models, and their effects were compared to select suitable stem cells for cell replacement stroke therapy. The rat and mice ischaemic models were constructed using the middle cerebral artery occlusion technique. Both electrocoagulation of the artery and the intraluminal filament technique were used. The behaviour changes and fates of grafted stem cells were determined mainly by behaviour testing and immunocytochemistry. Following iPSC transplantation into the corpora striata of normal mice, a tumour developed in the brain. The iNSCs survived well and migrated towards the injured area without differentiation. Although there was no tumourigenesis in the brain of normal or ischaemic mice after the iNSCs were transplanted in the cortices, the behaviour in ischaemic mice was not improved. Upon transplanting MSC and RMNE6 cells into ischaemic rat brains, results similar to iNSCs in mice were seen. However, transplantation of RMNE6 caused a brain tumour. Thus, tumourigenesis and indeterminate improvement of behaviour are challenging problems encountered in stem cell therapy for stroke, and the intrinsic characteristics of stem cells should be remodelled before transplantation. Copyright (c) 2015 John Wiley & Sons, Ltd.
基金：
the Major State Basic Research Development Program of China (973 Program; No.2006CB500700 and No. 2011CB504100),
the National Natural Science Foundation of China (No. 30430280)
the Beijing Committee of Science and Technology (No.Z0005187040311).
第一作者机构：[1]Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China[2]Beijing Center of Neural Regeneration and Repair, Capital Medical University, Beijing, China[3]Department of Neurobiology, Capital Medical University, Beijing, China[4]Beijing Key Laboratory of Major Brain Disorders, Capital Medical University, Beijing, China[5]Beijing Municipal Key Laboratory for Neural Regeneration and Repairing, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[*1]Department of Neurobiology, School of Basic sciences, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Beijing Center of Neural Regeneration and Repair, Capital Medical University, No. 10 Xitoutiao, Youanmen, Beijing 100069, China.
推荐引用方式(GB/T 7714)：
Yun Wu,Jianyu Wu,Rongkai Ju,et al.Comparison of intracerebral transplantation effects of different stem cells on rodent stroke models[J].CELL BIOCHEMISTRY AND FUNCTION.2015,33(4):174-182.doi:10.1002/cbf.3083.
APA：
Yun Wu,Jianyu Wu,Rongkai Ju,Zhiguo Chen&Qunyuan Xu.(2015).Comparison of intracerebral transplantation effects of different stem cells on rodent stroke models.CELL BIOCHEMISTRY AND FUNCTION,33,(4)
MLA：
Yun Wu,et al."Comparison of intracerebral transplantation effects of different stem cells on rodent stroke models".CELL BIOCHEMISTRY AND FUNCTION 33..4(2015):174-182
