机构：[1]Department of Neurology, Affiliated Hospital of Tai Shan Medical University, Taishan 271000, China[2]Department of Intensive Care Unit , 2nd Affiliated Hospital of Baotou Medical College, Baotou 014030, China[3]Department of Medicine, University of Arizona, Tucson, AZ 85721, USA[4]Biomedicine Research Center and Basic Medical College, Baotou Medical College, Baotou 014060, China[5]Department of Anatomy, University of California, San Francisco, CA 94143, USA[6]Institute for Hypoxia Medicine, Xuanwu Hospital of Capital Medical University, Beijing 10054,China首都医科大学宣武医院
It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1 alpha. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretreatment and normal saline (NS), respectively injected with CoCl2 and NS 3 h before exposure to hypoxia for 0 run (H0), 1 run (H1), and 4 runs (H4). Western Blot, electrophoretic mobility shift assay (EMSA), extracellular recordings population spikes in area cornus ammonis I (CA 1) of mouse hippocampal slices and real-time were used in this study. Our results demonstrated that the tolerance of mice to hypoxia, the changes of HIF-1 alpha protein level and HIF-1 DNA binding activity in mice hippocampus, the mRNA level of erythropoietin (EPO) and vascular endothelial growth factor (VEGF), and the disappearance time of population spikes of hippocampal slices were substantially different between the control group and the CoCl2 group. Over-induction of HIF-1 alpha by pretreatment with CoCl2 before hypoxia did not increase the hypoxia tolerance.
基金：
the National Natural Science Foundation of China(Nos. 39670271, 81060212, 81160244, 81360316),
Beijing Natural Science Foundation (No. 7962009),
China Postdoctoral Science Foundation (No. 20080430851),
Inner Mongolia Science Foundation (No. 2010BS1104),
Inner Mongolia Educational Research Foundation (No. NJ06011,No. NJZY12221, No. NJZY12225),
Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region (No. NJYT-13-A10).
第一作者机构：[1]Department of Neurology, Affiliated Hospital of Tai Shan Medical University, Taishan 271000, China
共同第一作者：
通讯作者：
通讯机构：[4]Biomedicine Research Center and Basic Medical College, Baotou Medical College, Baotou 014060, China[6]Institute for Hypoxia Medicine, Xuanwu Hospital of Capital Medical University, Beijing 10054,China
推荐引用方式(GB/T 7714)：
Yan-Bo Zhang,Xiulian Wang,Edward A. Meister,et al.The Effects of CoCl2 on HIF-1 alpha Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models[J].INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.2014,15(6):10999-11012.doi:10.3390/ijms150610999.
APA：
Yan-Bo Zhang,Xiulian Wang,Edward A. Meister,Ke-Rui Gong,Shao-Chun Yan...&Guo Shao.(2014).The Effects of CoCl2 on HIF-1 alpha Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,15,(6)
MLA：
Yan-Bo Zhang,et al."The Effects of CoCl2 on HIF-1 alpha Protein under Experimental Conditions of Autoprogressive Hypoxia Using Mouse Models".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 15..6(2014):10999-11012
