机构：[a]Department of Otolaryngology Head and Neck Surgery,Xuanwu Hospital, Capital Medical University, Beijing , China耳鼻咽喉-头颈外科首都医科大学宣武医院[b]Central Laboratory of Surgery, Xuanwu Hospital, Capital Medical University, Beijing , China首都医科大学宣武医院
Background: Mast cells (MCs) in the nasal respiratory mucosa (NRM) play a triggering role in the pathogenesis of allergic rhinitis (AR). Recent research evidence in mouse models of AR suggests an underlying MC-related allergic response in mouse nasal olfactory mucosa (NOM). Objective: We sought to investigate the phenotypic characteristics of nasal MCs in a mouse model of AR. Methods: By MC-specific staining and immunohistochemistry, we analyzed the subset, protease and IgE-binding phenotypes of nasal MCs in ovalbumin (OVA)-sensitized unchallenged and challenged mice. Results: In OVA-sensitized challenged mice, increased serum OVA-specific IgE levels (p < 0.001) and eosinophil infiltration confirmed AR induction. In addition to constitutive connective tissue MCs, mucosal MCs were induced in NRM and NOM of OVA-sensitized challenged mice. Connective tissue MCs and mucosal MCs in mouse NRM and NOM were positive for mouse MC protease-1, -4, -5, -6, -7 and carboxypeptidase-A3. In line with MCs in NRM, there were increased numbers (p = 0.019) and proportions (p = 0.027) of MCs with surface-bound IgE in NOM of OVA-sensitized challenged mice. Conclusion: In the setting of AR, MCs in mouse NOM exhibit the same subset, protease and IgE-binding phenotypes as MCs in mouse NRM. (C) 2014 S. Karger AG, Basel
基金：
the National Natural Science Foundation of China(81170894),
Beijing Natural Science Foundation (7113156),
Specialized Project on Scientific Research within the Healthcare Circle by the National Health and Family Planning Commission of China (201202005)
Key Discipline Construction Project of the Finance Bureau of Beijing (3500-114235).
第一作者机构：[a]Department of Otolaryngology Head and Neck Surgery,Xuanwu Hospital, Capital Medical University, Beijing , China
通讯作者：
通讯机构：[*1]Department of Otolaryngology Head and Neck Surgery Xuanwu Hospital, Capital Medical University No. 45 Changchun Street, Xi Cheng District, Beijing 100053 (China)
推荐引用方式(GB/T 7714)：
Pu Li,Yeqing Cui,Gongru Song,et al.Phenotypic Characteristics of Nasal Mast Cells in a Mouse Model of Allergic Rhinitis[J].ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery.2014,76(6):303-313.doi:10.1159/000369142.
APA：
Pu Li,Yeqing Cui,Gongru Song,Zhenlin Wang&Qiuhang Zhang.(2014).Phenotypic Characteristics of Nasal Mast Cells in a Mouse Model of Allergic Rhinitis.ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery,76,(6)
MLA：
Pu Li,et al."Phenotypic Characteristics of Nasal Mast Cells in a Mouse Model of Allergic Rhinitis".ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery 76..6(2014):303-313
