机构：[1]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China,首都医科大学宣武医院[2]Department of Anatomy, Anhui Medical University, Hefei, 230032, China,[3]Department of neurology, Northern Jiangsu People’s Hospital, Jiangsu, 225001, China.
Glial cell-derived neurotrophic factor (GDNF) has shown beneficial effects in models of Parkinson's disease. The mild results observed in the double-blind clinical trial by intraputamenal infusion of recombinant GDNF proteins warrant a search for alternative delivery methods. In this study, we investigated the function of autologous mesenchymal stem cells (MSCs) expressing GDNF (GDNF-MSCs) for protection against MPTP-induced injury in cynomolgus monkeys. MSCs were obtained from the bone marrow of individual monkeys and gene-modified to express GDNF. Following unilateral engraftment of GDNF-MSCs into the striatum and substantia nigra, the animals were challenged with MPTP to induce a stable systemic Parkinsonian state. The motor functions were spared in the contralateral limbs of monkeys receiving GDNF-MSCs, but not in those receiving MSCs alone. In the striatum of the grafted hemisphere, dopamine levels were higher and dopamine uptake was enhanced. The results suggest that autologous MSCs may be a safe vehicle to deliver GDNF for enhancing nigro-striatum functions.
基金：
the National Basic Research Program of China (2012CBA01307 and 2011CB965103)
National High Technology Research and Development Program of China (2011AA020106)
National Natural Science Foundation of China (31070946, 81141014)
High Level Talent Fund of the Beijing Healthcare System(2009-2-14)
Research Assistance Fund of Anhui Medical University (Grant No.XJ201008)
第一作者机构：[1]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China,[2]Department of Anatomy, Anhui Medical University, Hefei, 230032, China,
共同第一作者：
通讯作者：
通讯机构：[1]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China,[2]Department of Anatomy, Anhui Medical University, Hefei, 230032, China,
推荐引用方式(GB/T 7714)：
Zhenhua Ren,Jiayin Wang,Shuyan Wang,et al.Autologous transplantation of GDNF-expressing mesenchymal stem cells protects against MPTP-induced damage in cynomolgus monkeys[J].SCIENTIFIC REPORTS.2013,3:doi:10.1038/srep02786.
APA：
Zhenhua Ren,Jiayin Wang,Shuyan Wang,Chunlin Zou,Xiaobo Li...&Y. Alex Zhang.(2013).Autologous transplantation of GDNF-expressing mesenchymal stem cells protects against MPTP-induced damage in cynomolgus monkeys.SCIENTIFIC REPORTS,3,
MLA：
Zhenhua Ren,et al."Autologous transplantation of GDNF-expressing mesenchymal stem cells protects against MPTP-induced damage in cynomolgus monkeys".SCIENTIFIC REPORTS 3.(2013)
