机构：[1]Protein Science Laboratory of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing, China,[2]College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China,[3]Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, Zhejiang, China,[4]Xuanwu Hospital, Capital Medical University, Beijing, China,首都医科大学宣武医院[5]Department of Surgery, Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America
Background: EMX2 is a human orthologue of the Drosophila empty spiracles homeobox gene that has been implicated in embryogenesis. Recent studies suggest possible involvement of EMX2 in human cancers; however, the role of EMX2 in carcinogenesis needs further exploration. Results: In this study, we reported that down-regulation of EMX2 expression was significantly correlated with EMX2 promoter hypermethylation in gastric cancer. Restoring EMX2 expression using an adenovirus delivery system in gastric cancer cell lines lacking endogenous EMX2 expression led to inhibition of cell proliferation and Wnt signaling pathway both in vitro and in a gastric cancer xenograft model in vivo. In addition, we observed that animals treated with the adenoviral EMX2 expression vector had significantly better survival than those treated with empty adenoviral vector. Conclusion: Our study suggests that EMX2 is a putative tumor suppressor in human gastric cancer. The adenoviral-EMX2 may have potential as a novel gene therapy for the treatment of patients with gastric cancer.
基金：
National Key Basic Research and Development (973) Program of China [2011CB910800, 2012CB917304]; National Natural Science Foundation of China [31170732]; Natural Science Foundation of Zhejiang Province [Y2101329]; Postdoctoral Science Foundation of Zhejiang Province [2010-BSH-031]
第一作者机构：[1]Protein Science Laboratory of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing, China,
通讯作者：
通讯机构：[1]Protein Science Laboratory of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing, China,[3]Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, Zhejiang, China,[5]Department of Surgery, Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America
推荐引用方式(GB/T 7714)：
Jie Li,MinliMo,Zhao Chen,et al.Adenoviral Delivery of the EMX2 Gene Suppresses Growth in Human Gastric Cancer[J].PLOS ONE.2012,7(9):doi:10.1371/journal.pone.0045970.
APA：
Jie Li,MinliMo,Zhao Chen,Zhe Chen,Qing Sheng...&Hai-Meng Zhou.(2012).Adenoviral Delivery of the EMX2 Gene Suppresses Growth in Human Gastric Cancer.PLOS ONE,7,(9)
MLA：
Jie Li,et al."Adenoviral Delivery of the EMX2 Gene Suppresses Growth in Human Gastric Cancer".PLOS ONE 7..9(2012)
