机构：[1]Department of Traditional Chinese Medicine, Xuanwu Hospital of Capital Medical University, Beijing 100053, People’s Republic of China首都医科大学宣武医院[2]Department of Pharmacology, Xuanwu Hospital of Capital Medical University, No. 45, Chang-Chun Street, Beijing 100053, People’s Republic of China首都医科大学宣武医院
The present study was to investigate the influence of tenuigenin, an active ingredient of Polygala tenuifolia Willd, on the proliferation and differentiation of hippocampal neural stem cells in vitro. Tenuigenin was added to a neurosphere culture and neurosphere growth was measured using MTT assay. The influence of tenuigenin on the proliferation of neural progenitors was examined by Clone forming assay and BrdU detection. In addition, the differentiation of neural stem cells was compared using immunocytochemistry for beta III-tubulin and GFAP. The results showed that addition of tenuigenin to the neural stem cell medium increased the number of newly formed neuro-spheres. More neurons were also obtained when tenuigenin was added in the differentiation medium. These findings suggest that tenuigenin is involved in regulating the proliferation and differentiation of hippocampal neural stem cells. This result may be one of the underlying reasons for tenuigenin's nootropic and anti-aging effects.
基金：
National Natural Science Foundation of China (81102624 and 30973513)
Beijing Natural Science Foundation (7102073)
Beijing Key Subject Discipline of Integrated Traditional and Western Medicine (VI 26)
第一作者机构：[1]Department of Traditional Chinese Medicine, Xuanwu Hospital of Capital Medical University, Beijing 100053, People’s Republic of China
通讯作者：
通讯机构：[2]Department of Pharmacology, Xuanwu Hospital of Capital Medical University, No. 45, Chang-Chun Street, Beijing 100053, People’s Republic of China
推荐引用方式(GB/T 7714)：
Yujing Chen,Xiaobo Huang,Wenqiang Chen,et al.Tenuigenin Promotes Proliferation and Differentiation of Hippocampal Neural Stem Cells[J].NEUROCHEMICAL RESEARCH.2012,37(4):771-777.doi:10.1007/s11064-011-0671-3.
APA：
Yujing Chen,Xiaobo Huang,Wenqiang Chen,Ningqun Wang&Lin Li.(2012).Tenuigenin Promotes Proliferation and Differentiation of Hippocampal Neural Stem Cells.NEUROCHEMICAL RESEARCH,37,(4)
MLA：
Yujing Chen,et al."Tenuigenin Promotes Proliferation and Differentiation of Hippocampal Neural Stem Cells".NEUROCHEMICAL RESEARCH 37..4(2012):771-777
