Seropositivity rates of water channel protein 4 antibodies compared between a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay in neuromyelitis optica patients
机构：[1]Department of Neurology and Key Laboratory Ministry of Education on Neurodegenerative Diseases, Xuanwu Hospital of Capital Medical University, Beijing 100053, China神经内科首都医科大学宣武医院[2]Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing 100053, China神经内科首都医科大学宣武医院
A total of 66 samples (from 27 cases with neuromyelitis optica, 26 cases with multiple sclerosis, and 13 cases with optic neuritis) were tested for aquaporin-4 antibody by a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay. The sensitivities and specificities of the two assays were similar. We further analyzed an additional 68 patients and 93 healthy controls using the enzyme-linked immunosorbent assay. A Kappa test showed good consistency between the two methods in terms of detection of anti-aquaporin-4 antibody in the sera of neuromyelitis optica patients. No significant correlations were identified with onset age or disease duration, suggesting that aquaporin-4 antibody is a good marker for neuromyelitis optica. The enzyme-linked immunosorbent assay can be used for quantifying aquaporin-4 antibody concentrations and may be useful to dynamically monitor changes in the levels of aquaporin-4 antibody during disease duration.
基金：
the grants from the Ministry of Sciences and Technology of China, No. 2006AA02A408*, 2008ZX09312-014*
第一作者机构：[1]Department of Neurology and Key Laboratory Ministry of Education on Neurodegenerative Diseases, Xuanwu Hospital of Capital Medical University, Beijing 100053, China
通讯作者：
通讯机构：[1]Department of Neurology and Key Laboratory Ministry of Education on Neurodegenerative Diseases, Xuanwu Hospital of Capital Medical University, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Xiaoli Wu,Zhangyuan Liao,Jing Ye,et al.Seropositivity rates of water channel protein 4 antibodies compared between a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay in neuromyelitis optica patients[J].NEURAL REGENERATION RESEARCH.2011,6(32):2490-2494.doi:10.3969/j.issn.1673-5374.2011.32.002.
APA：
Xiaoli Wu,Zhangyuan Liao,Jing Ye,Huiqing Dong,Chaodong Wang&Piu Chan.(2011).Seropositivity rates of water channel protein 4 antibodies compared between a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay in neuromyelitis optica patients.NEURAL REGENERATION RESEARCH,6,(32)
MLA：
Xiaoli Wu,et al."Seropositivity rates of water channel protein 4 antibodies compared between a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay in neuromyelitis optica patients".NEURAL REGENERATION RESEARCH 6..32(2011):2490-2494
