机构：[1]Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, San Francisco, California[2]Department of Neurosurgery, Xuanwu Hospital, Capital University of Medical Sciences, Beijing, China神经外科首都医科大学宣武医院[3]Department of Neurological Surgery, University of California, San Francisco, San Francisco, California[4]Med-X Research Institute, Shanghai JiaoTong University, Shanghai, China[5]Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, Departments of Neurological Surgery and Neurology, University of California, San Francisco, San Francisco, California
BACKGROUND: Brain and spinal cord arteriovenous malformations (AVMs) are characterized by aberrant angiogenesis and vascular remodeling. Endothelial progenitor cells (EPCs) can be recruited by stromal cell-derived factor-1 (SDF-1), and participate in vascular remodeling in both physiological and pathological settings. OBJECTIVE: To investigate whether there are increased EPC levels in the brain and spinal cord AVM nidus. METHODS: Microsurgical specimens without endovascular embolization and radiosurgery from the brain (n = 12) and spinal cord (n = 5) AVMs were examined. Hemangioblastoma, meningioma, cerebral cortex obtained from epilepsy surgery, and the basilar artery from the autopsy were chosen for control comparisons. EPCs were identified as cells that were double-positive for the stem cell marker CD133 and the endothelial cell marker VEGFR-2 (vascular endothelial growth factor receptor-2 or KDR). In addition, SDF-1 was characterized by immunohistochemistry. RESULTS: Both brain and spinal AVM tissues displayed more CD133-, SDF-1-, and CD68-positive signals than epilepsy and basilar artery control tissues. The level of EPCs was increased in the brain and spinal cord AVM nidus, mainly at the edge of the vessel wall. The expression of SDF-1 was colocalized with CD31-positive and a-smooth muscle cells, and was predominantly found within the vessel wall. CONCLUSION: Our data demonstrate that EPCs are present in the nidus of the brain and spinal cord AVMs, which may mediate pathological vascular remodeling and impact the clinical course of AVMs.
基金：
National Institutes of Health grants R01 NS27713，R01 NS34949 ，P01 NS44155 ，and R03 NS067507
第一作者机构：[1]Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, San Francisco, California[2]Department of Neurosurgery, Xuanwu Hospital, Capital University of Medical Sciences, Beijing, China
通讯作者：
通讯机构：[*]Department of Anesthesia and Perioperative Care, University of California, San Francisco, 1001 Potrero Avenue, Room 3C-38, San Francisco, CA 94110.
推荐引用方式(GB/T 7714)：
Peng Gao,Yongmei Chen,Michael T. Lawton,et al.Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations[J].NEUROSURGERY.2010,67(4):1029-1035.doi:10.1227/NEU.0b013e3181ecc49e.
APA：
Peng Gao,Yongmei Chen,Michael T. Lawton,Nicholas M. Barbaro,Guo-Yuan Yang...&William L. Young.(2010).Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations.NEUROSURGERY,67,(4)
MLA：
Peng Gao,et al."Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations".NEUROSURGERY 67..4(2010):1029-1035
医学