Reverse line blot hybridization and DNA sequencing studies of the 16S-23S rRNA gene intergenic spacer regions of five emerging pathogenic Nocardia species
机构：[1]Department of Dermatology and Venerology, Xuan Wu Hospital, Capital Medical University, Beijing, PR China首都医科大学宣武医院[2]Department of Dermatology and Venerology, The Affiliated Hospital of Inner Mongolia Medical College, Hohhot, PR China[3]Centre for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia[4]Life Science College, Peking University, Beijing, PR China[5]Department of Dermatology, Tianjin Medical University General Hospital, Tianjin, PR China[6]Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute, The University of Sydney, Westmead, New South Wales, Australia
The objective of this study was to examine DNA sequence polymorphisms in the 16S-23S rRNA gene intergenic spacer (ITS) regions of five emerging pathogenic Nocardia species Nocardia beijingensis, Nocardia blacklockiae, Nocardia tharlandica, Nocardia elegans and Nocardia vinacea A set of six isolates belonging to the species of interest and 135 isolates belonging to other Nocardia species was studied A PCR-based reverse line blot (RLB) hybridization assay incorporating species- or intraspecies ITS rRNA gene operon-specific probes was then developed for species identification Substantial intraspecies sequence variation among different ITS operons was identified Four sequence types of N tharlandica. eight sequence types of N beijingensis (four types for each of two strains) and five sequence types of N blacklockiae, N elegans and N vinacea were found. The results represent the first evidence of ITS sequence heterogeneity in emerging species of Nocardia By incorporating species/operon-specific probes into a RLB assay, unique RLB patterns were identified for each of the species and every sequence type The PCR/RLB assay demonstrated high specificity and showed promise in both the identification and genotyping of Nocardia species More detailed studies of the polymorphism within the ITS locus may further advance our capacity to reliably identify and subtype medically important Nocardia species
第一作者机构：[1]Department of Dermatology and Venerology, Xuan Wu Hospital, Capital Medical University, Beijing, PR China[2]Department of Dermatology and Venerology, The Affiliated Hospital of Inner Mongolia Medical College, Hohhot, PR China[3]Centre for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia
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通讯作者：
通讯机构：[3]Centre for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia
推荐引用方式(GB/T 7714)：
Xiaoyan Wang ,Meng Xiao ,Fanrong Kong ,et al.Reverse line blot hybridization and DNA sequencing studies of the 16S-23S rRNA gene intergenic spacer regions of five emerging pathogenic Nocardia species[J].JOURNAL OF MEDICAL MICROBIOLOGY.2010,59(5):548-555.doi:10.1099/jmm.0.017921-0.
APA：
Xiaoyan Wang,,Meng Xiao,,Fanrong Kong,,Vitali Sintchenko,,Huiping Wang,...&Sharon Chen.(2010).Reverse line blot hybridization and DNA sequencing studies of the 16S-23S rRNA gene intergenic spacer regions of five emerging pathogenic Nocardia species.JOURNAL OF MEDICAL MICROBIOLOGY,59,(5)
MLA：
Xiaoyan Wang,,et al."Reverse line blot hybridization and DNA sequencing studies of the 16S-23S rRNA gene intergenic spacer regions of five emerging pathogenic Nocardia species".JOURNAL OF MEDICAL MICROBIOLOGY 59..5(2010):548-555
