AIM: To investigate the effects of hyperlipidemia on acute pancreatitis (AP) and the possible mechanisms. METHODS: Rat models of hyperlipidemia and AP were established by Triton WR1339 and cerulein respectively. Human albumin was used to treat AP complicated by hyperlipidemia. In each group, we compared the histological score, volume of ascites, ratio of pancreatic wet/dry weight, serum amylase (AMY) and pancreatic acinar cell apoptosis. The level of protein kinase C (PKC) membrane translocation in pancreatic tissue was detected by Western blot. RESULTS: In the hyperlipidemia model established by Triton WR1339, triglyceride (TG) increased remarkably and reached its peak 6 h after injection, and most rats developed mild acute pancreatitis. Histological score, volume of ascites, ratio of wet/dry weight and serum AMY in AP animals with hyperlipidemia were obviously higher than those in AP animals (P < 0.05) and decreased after albumin therapy but not significantly (P > 0.05). Apoptotic cells detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) increased in AP animals with hyperlipidemia and did not change distinctly after albumin therapy. PKC membrane translocation level increased in AP animals with hyperlipidemia and decreased remarkably after albumin therapy (P < 0.05). CONCLUSION: Hyperlipidemia may induce AP or intensify pancreatic injury. Albumin therapy can not alleviate pancreatic lesion effectively. PKC activation may be one mechanism by which AP is intensified by hyperlipidemia. (C) 2006 The WJG Press. All rights reserved.
基金：
Supported by the Key Program of Beijing Academy of Health,
No.1998-11-2001-09
第一作者机构：[1]Department of General Surgery, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China[*1]Department of General Surgery, Xuanwu Hospital of Capital University of Medical Sciences, 45 Changchun Street, Beijing 100053, China.
通讯作者：
通讯机构：[*1]Department of General Surgery, Xuanwu Hospital of Capital University of Medical Sciences, 45 Changchun Street, Beijing 100053, China.
推荐引用方式(GB/T 7714)：
Ya-Jun Wang,Jia-Bang Sun,Fei Li,et al.Hyperlipidemia intensifies cerulein-induced acute pancreatitis associated with activation of protein kinase C in rats[J].WORLD JOURNAL OF GASTROENTEROLOGY.2006,12(18):2908-2913.doi:10.3748/wjg.v12.i18.2908.
APA：
Ya-Jun Wang,Jia-Bang Sun,Fei Li&Shu-Wen Zhang.(2006).Hyperlipidemia intensifies cerulein-induced acute pancreatitis associated with activation of protein kinase C in rats.WORLD JOURNAL OF GASTROENTEROLOGY,12,(18)
MLA：
Ya-Jun Wang,et al."Hyperlipidemia intensifies cerulein-induced acute pancreatitis associated with activation of protein kinase C in rats".WORLD JOURNAL OF GASTROENTEROLOGY 12..18(2006):2908-2913
