机构：[a]Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan[b]Department of Orthopaedic Surgery, Xuan Wu Hospital, Capital University of Medical Sciences, No. 45 Changchunjie, Xuanwu District, Beijing, 100053, China首都医科大学宣武医院[c]Section of Orthopedic Surgery, Division of Bio-Matrix, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan[d]CREST JST (Japan Science and Technology), Japan[e]COE Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan
To obtain more extensive bone formation in composites of porous ceramics and bone marrow stromal cells (BMSCs), we hypothesized that a low-pressure system would serve to facilitate the perfusion of larger number of BMSCs into the porous scaffold, enhancing bone formation within the composites. After culturing BMSCs in osteogenic medium, porous blocks of beta-tricalcium phosphate (beta-TCP) were soaked in the cell suspension. Composites of the block and BMSCs were put immediately into a vacuum desiccator. Low pressure was applied to the low pressure group, while controls were left at atmospheric pressure. Composites were incubated in vitro or subcutaneously implanted into syngeneic rats, then analyzed biologically and histologically. In the in vitro group, cell suspension volume, cell seeding efficiency, alkaline phosphatase (ALP) activity, and DNA content in the beta-TCP blocks were significantly higher in low pressure group than in the controls. Scanning electron microscopy (SEM) demonstrated that a greater number of cells covered the central parts of the composites in the low pressure group. ALP activity in the composites was increased at 3 and 6 weeks after implantation into rats. Histomorphometric analysis revealed more uniform and extensive bone formation in the low pressure group than in the controls. The application of low pressure during the seeding of BMSCs in perfusing medium into a porous scaffold is useful for tissue-engineered bone formation. (c) 2005 Elsevier Ltd. All rights reserved.
第一作者机构：[a]Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan[b]Department of Orthopaedic Surgery, Xuan Wu Hospital, Capital University of Medical Sciences, No. 45 Changchunjie, Xuanwu District, Beijing, 100053, China
通讯作者：
通讯机构：[a]Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan
推荐引用方式(GB/T 7714)：
Juyong Wang,Yoshinori Asou,Ichiro Sekiya,et al.Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold[J].BIOMATERIALS.2006,27(13):2738-2746.doi:10.1016/j.biomaterials.2005.12.005.
APA：
Juyong Wang,Yoshinori Asou,Ichiro Sekiya,Shinichi Sotome,Hisaya Orii&Kenichi Shinomiya.(2006).Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold.BIOMATERIALS,27,(13)
MLA：
Juyong Wang,et al."Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold".BIOMATERIALS 27..13(2006):2738-2746
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