机构:[1]Natl Res Inst Family Planning, Dept Mol Biol, Beijing 100081, Peoples R China[2]Beijing Xuanwu Hosp, Dept Obstet & Gynecol, Beijing, Peoples R China首都医科大学宣武医院[3]Natl Res Inst Family Planning, Dept Mol Biol, 12 Da Hui Si, Beijing 100081, Peoples R China
OBJECTIVE: To detect human parvovirus B19 intrauterine infection in pregnancy with the polymerase chain reaction (PCR). STUDY DESIGN: DNA of chorionic villi and amniotic fluid was extracted and the gene of human parvovirus B19 amplified with PCR. RESULTS: The study analyzed 61 specimens of chorionic villi and 26 specimens of amniotic fluid and found two positive specimens of chorionic villi and I positive specimen of amniotic fluid. CONCLUSION: The vertical transmission of human parvovirus B19 infection in early pregnancy may be a pathway of intrauterine infection. Chorionic villus sampling in early pregnancy and PCR could be developed as a method of prenatal diagnosis of human parvovirus B19 intrauterine infection.
第一作者机构:[1]Natl Res Inst Family Planning, Dept Mol Biol, Beijing 100081, Peoples R China[2]Beijing Xuanwu Hosp, Dept Obstet & Gynecol, Beijing, Peoples R China[3]Natl Res Inst Family Planning, Dept Mol Biol, 12 Da Hui Si, Beijing 100081, Peoples R China
通讯作者:
通讯机构:[3]Natl Res Inst Family Planning, Dept Mol Biol, 12 Da Hui Si, Beijing 100081, Peoples R China
推荐引用方式(GB/T 7714):
Dong ZW,Zhou SY,Li Y,et al.Detection of a human parvovirus intrauterine infection with the polymerase chain reaction[J].JOURNAL OF REPRODUCTIVE MEDICINE.2000,45(5):410-412.
APA:
Dong, ZW,Zhou, SY,Li, Y&Liu, RM.(2000).Detection of a human parvovirus intrauterine infection with the polymerase chain reaction.JOURNAL OF REPRODUCTIVE MEDICINE,45,(5)
MLA:
Dong, ZW,et al."Detection of a human parvovirus intrauterine infection with the polymerase chain reaction".JOURNAL OF REPRODUCTIVE MEDICINE 45..5(2000):410-412